The Preliminary Study Of Umbilical Cord-derived Mesenc Hymal Stem Cells Carrying Reovirus Which Killed Leukemi A Cell Lines In Vitro

Objective: In the present of Reo3 antibodies like the internal environment of human, the experiment explored the feasibility of using human umbilical cord mesenchymal stem cells(UC-MSCs) to carry Reovirus Type 3 Dearing(Reo3) and the effect of apoptosis and necrosis on leukemia cell line Jurkat cells which is co-cultured with Jurkat cells in vitro for providing basic research for the treatment of leukemia and other cancers. Methods:1.The isolated, culture, and identification of UC-MSCs; 2.The adhesion molecules of JAM-A expression on UC-MSCs; 3.After infected with high titer of Reo3, the internal change of UC-MSCs were observed; 4.The relative proliferation rate of UC-MSCs after infected with Reo3;5.The titers of Reo3 in UC-MSCs Lysates were determined after which were infected with Reo3; 6.The establishment of co-culture system in vitro and the analysis of apoptosis and necrosis of Jurkat cells in the system. Results:1.The cells isolated from human umbilical cord Wharton’s jelly by mechanical methods were in line with the biological characteristics of UC-MSCs by morphology and cell surface antigen detection; 2.The surface adhesion molecule of JAM-A expression of UC-MSCs and Jurkat cells were 50.7%, and 41.3% respectively; 3. After UC-MSCs infected with high viral titer Reo3 for 72 hours, a large number of viral inclusions appeared in UC-MSCs whlie no viral inclusions occured inside UC-MSCs uninfected; 4 compared with no Reo3 group, the relative growth rates of UC-MSCs with Reo3 of MOI 1, MOI2 and MOI 3 exhibited rise and decline within 24-120 hours, and both reached peak points at 72 hours. At the 120 hours, it had no significantly statistically than no Reo3 group exclusively. So the best infection titer of Reo3 was degreed as MOI 1; 5.Because of the highest titer of Reo3 in UC-MSCs lysate was at 48 hours, thetime-point of infection was set at 48 hours; 6 after the establishment of co-culture system for 24 hours with the above conditions, It showed that UC-MSCs carried with Reo3 still can cause apoptosis and necrosis on Jurkat cells in the presence of antibodies. Conclusion: 1.The cells isolated from human umbilical cord Wharton’s jelly were in line with the biological characteristics of UC-MSCs by detection and identification of the morphology and molecular markers of cell surface.2.It confirmed that UC-MSCs can be used as a cell carrier for Reo3; 3.In the presence of antibodies,UC-MSCs carrying with Reo3 can lead Jurkat cells to apoptosis and necrosis; 4.It has some theoretical basis of Reo3 as a new drug for cancer therapy.