Ferulic Acid’s Protective Effect Ion On Brain Microvascular Endothelial Cells Caused By Oxygen-glucose Deprivation

ObjectiveWe want To find out the protective effects of ferulic acid (FA) on oxidative injury of brain microvascular endothelial cells (BMEC) induced by oxygen-glucose deprivation (OGD) at different time points.MethodsWe carried out the experiment at the cellular level, using mixed gas (95% nitrogen+5% carbon dioxide) to establish OGD model in vitro. Established the groups in accordance to the experimental purpose into normal group, model group and the group of ferulic acid (FA). First we used the cell activity determined by MTT method. The model’s cell activity compared to normal group have significant differences, suggesting that we successfully established the model. Using flow cytometry detection technology, detecting the reactive oxygen species (reactive oxygen species, ROS) quantity, excessive ROS hints of cumulative damage cells oxidative damage degree, detection of ROS production directly reflect the levels of oxidative damage on cells. Then detecting the cell malondialdehyde (methane dicarboxylic aldehyde, MDA) level, MDA is one of the important product of membrane lipid oxidation, the level of MDA also prompted the levels of oxidative damage on cells. Using nitroblue tetrazolium chromogenic method, detected the cell total superoxide dismutase (superoxide dismutase, SOD) activity. SOD is one of the widely exists in animals and plants important antioxidant enzymes, the activity of SOD prompts the antioxidant capacity of the cells.ResultsThe BMEC s damage which caused by OGD was associate with time manner. According to the comparison to the OGD group, FA can increased the BMEC’ notablely viability, it can also decline ROS generation and decrease MDA level, on the other hand, increased the SOD level.ConclusionFA can notable protect BMEC from the impaired by OGD at different time points. The potential mechanism may be associated with the amelioration of OGD-induced oxidative stress and damage.