The Vitro Study Of Duraphat Fluoride Varnish On Remineralization Of Demineralized Permanent Enamel

Objective To explore and provide the clinical reference of Duraphat fluoride varnish on remineralization of demineralized permanent enamel by carbonated beverages.Methods 1.The vitro study of demineralization of permanent enamel by carbonated beverage.15 permanent premolar teeth from 12 to 25 years old young people were collected from orthodontic extraction.2 enamel blocks were cut from each tooth.2 enamel blocks were randomly divided into group A and group B. And each group had 15 blocks. Group A were immersed in cola carbonated beverage in 10 min,4 times a day for 7 days at 37. Group B, as the control group, were immersed in deionized water in 10 min,4 times a day for 7 days at 37℃. The changes of enamel surface morphology were observed by scanning electron microscope. And the changes of Ca, P content(weight percentage) were detected by energy spectrum analyzer, the difference of the two groups were analyzed by LSD-t test.2.The vitro study of Duraphat fluoride varnish on remineralization of demineralized permanent enamel by carbonated beverage.15 permanent premolar teeth from 12 to 25 years old young people were collected from orthodontic extraction.3 enamel blocks were cut from each tooth.3 enamel blocks were randomly divided into group A, group B and group C. And each group had 15 blocks. Group A:15 enamel blocks treated in cola in 10 min,4 times a day for 7 days at 37℃+Duraphat fluoride varnish. Group B:15 enamel blocks treated in cola in 10 min,4 times a day for 7 days at 37℃ +2%NaF solution. Group C:15 enamel blocks treated in cola in 10 min,4 times a day for 7 days at 37℃, as the control. And all the enamel blocks were immersed in artificial saliva 7 days at 37℃. The changes of enamel surface morphology were observed by scanning electron microscope. And the changes of Ca, P content(weight percentage) were detected by energy spectrum analyzer, the difference of the three groups were analyzed by LSD-t test.Result 1.The vitro study of demineralization of permanent enamel by carbonated beverage. There were shallow depressions with irregular borders on the surface of permanent enamel of group A. The surfaces of the depressed areas showed a characteristic honeycomb-like appearance by SEM. By contrast, the surface of permanent enamel of group B were smooth flat. The values of the Ca, P content of group A were Ca:41.47±3.73, P:18.17±0.77. And the group B were Ca:45.16±3.67, P:18.67±0.44. The difference between group A and B was statistical significant(P<0.05).2.The vitro study of Duraphat fluoride varnish on remineralization of demineralized permanent enamel by carbonated beverage. There were characters of remineralization on the surface of all the permanent enamel samples by SEM. However, the characters of remineralization of group A were different from group B. Group A showed irregular large globule deposits on the surface. Group B showed some globule structures on the surface. Group C showed a little deposits on the surface. The values of the Ca, P content of group A were Ca:39.72±3.94, P:18.24±0.47, group B were Ca:39.54±2.40, P:17.70±0.71, and group C were Ca:36.85±2.87, P:17.70±0.71. Both the difference of Ca, P content between group A and C were statistical significant(P<0.05). The difference of Ca content between group B and C was statistical significant(P<0.05). The difference of P content between group B and C was not statistical significant(P>0.05). The difference of Ca, P content between group A and B were not statistical significant(P>0.05).Conclusion 1.The obvious permanent enamel demineralization can be caused by carbonated beverage in this vitro study.2.Duraphat fluoride varnish can promote the remineralization of demineralized permanent enamel by carbonated beverage in this vitro study.