The Effect Of VEGFR3-mediated Immune-nanoemulsion Of Ginsenoside Rg3 On Tumor Invasion, Metastasis And The Expression Of VEGF-C, TFG-β1 In Nude Mice Models Of Gastric Cancer

Objective:Establish nude mouse gastric cancer in situ fluorescence model, discusses the role of VEGFR3-mediated immune-nanoemulsion of ginsenoside Rg3 (VRIN) to gastric cancer nude mouse orthotopic transplantation tumor metastasis and its effect on Vascular endothelial growth factor C (VEGF-C), Transforming growth factor betal (TGF-β1) expression of gastric cancer in nude mice serum.Methods:Using stable cell lines expressing red fluorescent NUGC-4 gastric cancer cells in nude mice was successfully established fluorescence in situ neoplasia model.9 days after building,32 people gastric transplantation tumor nude mice were randomly divided into four groups, each group of 8, respectively to the normal saline,5-Fluorouracil, VRIN low doses, VRIN high doses to intervene, observation each group nude mouse survival ststus and tumor metastasis. End of the experiment, mice were sacrificed, anatomical open, check the tumor metastasis in an open fluorescence stereo microscope, the transfer rate is calculated, and specimens the orthotopic transplantation tumor and metastatic tumor tissue for pathological examination. Enzyme-Linked ImmunoSorbent Assay (ELISA) to detect nude mice serum VEGF-C, TGF-β1 expression.Result:1. Successfully established orthotopic gastric fluorescent model, and some of these mice had tumor metastasis, the transfer rate of normal saline group,5-FU group, VRIN low-dose group, VRIN high dose group were:87.5%,50%,37.5% and 12.5%. the difference had a statistical significance between VRIN high dose group and normal saline group (P<0.05).2. Compared with normal saline group,5-FU group, VRIN low dose group, VRIN high-dose group could significantly reduce the expression of gastric serum VEGF-C, TGF-β1, the difference had a significant statistical significance (P<0.05).Compared with VRIN low dose group, the 5-FU group and VRIN high-dose group can obviously reduce the expression of VEGF-C and TGF-β1, the difference had a significant statistical significance (P<0.05). the expression of VEGF-C and TGF-β1 between VRIN high dose group and 5-FU group had no significant difference (P> 0.05). the expression of VEGF-C and TGF-β1 in metastatic gastric cancer in nude mice group was significantly higher than that without metastasis (P<0.05).Conclusion:1. Using stable cell lines expressing red fluorescent NUGC-4 gastric cancer cells in nude mice can successfully establish fluorescence in situ neoplasia model. VEGFR3-mediated immune-nanoemulsion of ginsenoside Rg3 (VRIN) can significantly inhibit the gastric cancer nude mouse orthotopic transplantation tumor metastasis.2. The expression of VEGF-C and TGF-β1 is closely related to the metastasis of gastric cancer. VRIN can significantly down-regulate the level of VEGF-C and TGF-β1 in nude mice serum, which may be the mechanism of VRIN inhibiting gastric cancer metastasis.