The Expression Level Of AQP3 In Scleroderma Mouse Skin Fibroblast

Objective: Scleroderma is characterized by skin and internal organ fibrosis, Mainly involving escphagus, lung,kidney, heart and other organs. It may endanger the life at a late stage of disease. Now the pathogenesis of disease is unknow. When we refer to the literature at home and abroad, We have found that AQP3 may be the important molecules involving the mechanism. This research detect the role of AQP3 in sclerderma then find a new direction on therapy. Methods: We use different concentration of bleomycin subcutaneous injections to build the best scleroderma animal model in mice, then using the method of enzyme digestion and tissue block method to cultivate the original generation mice skin fibroblasts. At last, We use WB to dectect the expression level of AQP3 in scleroderma fibroblast. Results: Contrasted with the reference group(P<0.05), in which the mice were injected with phosphate buffer, the two parameters of dermishy- droxyproline content and dermis thickness were significantly higher for the experiment groups of 700μg/mL and 1000μg/mL bleomycin injection respectively. In addition, skin thicknesses of injective site were increased and skin elasticity was decreased for the mice of above given two experiment groups, whereas, the skin ulcer of injective site occured in the experiment group of 1000μg/mL bleomycin injection. The level of AQP3 in scleroderma mouse skin fibroblasts is higher than the normal ones. Conclusions: There was a close relationship between scleroderma mouse model and drug concentration. This paper proposed 700μg/mL bleomycin injection as the optimal drug concentrantion, in which the further study on pathogenesis of scleroderma and new therapeutic targets were provided reliable guarantee by the establishment of such a scleroderma mouse model. And the high level of AQP3 in scleroderma skin fibroblasts may be the crucial part of pathogenesis in scleroderma.