Study Of 3-MA’s Effect On Chemotherapy Drugs Inducing NK/T Cell Lymphoma Cell Lines To Apoptosis

Objective: NK/T cell lymphoma is a kind of malignant lymphoma which are most commonly located on in nasal and surrounding tissues. The patients’ prognosis is poorer.The patients have shorter lifetime,have poor sensitivity to conventional chemotherapy regimens,and are easy to relapse.Effective chemotherapy improve the CR rate and reduce the recurrence,especially for the patients with late or accompanied by nasal lesions.At present,the curative effect of the traditional chemotherapy regimens in conventional treatment is poor.Thats need to explore more effective drug treatment in order to improve the treatment efficiency and prolong patient survival. Methods: Cultivated NK/T cell lymphoma cells,grouped(A:NVB+EPI+3-MA,B:NVB+EPI,C:NVB+ADM+3-MA,D:NVB+ADM,E:TXT+EPI+3-MA,F:TXT+EPI,G:TXT+ADM+3-MA,H:TXT+ADM,I:3-MA,J:control),disposed cells,and collected cells after 24 hours.Extracted cell proteins after cracking cell,proceeding western blot test.Taking β-actin as reference,analysised gray levels by image J software to detecte autophagy proteins Beclin-1 and LC3 II expression;Collected different drug group of cells function for 24 hours,and used FCM to test cell apoptosis.Did these to study of autophagy inhibitor combination chemotherapy drugs inducing NK/T cell lymphoma cell lines to apoptosis,and provide laboratory evidence of the selection of clinical chemotherapy regimens for the NK/T cell lymphoma. Results: NVB,ADM,TXT,EPI and autophagy inhibitor 3- MA joint plans deal with NK/T cell tumor cells,autophagy protein expression in cells:chemotherapy drug group higher than the cell contrast group,3-MA group lower than the cell contrast group,chemotherapy drug group higher than Chemotherapy drugs combined 3- MA group,there are statistically significant differences in pair-wise comparison(P<0.05). FCM test cells apoptosis,the size of the cell apoptosis rate from high to low in turn:A、B、E、F、G、H、C、D、I、J.In bivariate scatter plot, the experimental group have different percentage of apoptotic cells in the right quadrant(Q2, 4).Compared with the cell contrast group there was statistically significant difference(P<0.05). Conclusion: When chemotherapy drug effects on NK/T cell lymphoma cell line, they can cause tumor cell protective autophagy, and autophagy related protein LC3 II and Beclin-1 expressing higher.3-MA can effective inhibition autophagy reaction of NK/T cell lymphoma cell,and autophagy related protein LC3 II and Beclin-1 expressing lower.3-MA can promote the chemotherapy drug induced NK/T cell lymphoma cells apoptosis.