Effects Of Apolipoprotein M On The Level Of Serum IL-10 And IL-6 In Mice

Objective: To evaluate the effect of apolipoprotein M(ApoM) on the level of serum interleukin-10 and interleukin-6 in inflammatory mice induced by lipopolysaccharide injection.Methods: Using base quenched probe techniques, we confirmed the genetypes of mice as ApoM wild type(ApoM+/+) or ApoM knockout type(ApoM-/-). We chose 12 mice each type with weight of 25 grams. Each type was divided into two groups, one group were injected with 100μl normal saline into their abdominal cavities every 24 hours for three times, the other group were injected with 100μl lipopolysaccharide(1.25mg/ml). Then we tested the concentration of interleukin-10 and interleukin-6 in advantage of liquid phase chip technology based on XMAP Luminex system.Results: Interleukin-10 and interleukin-6 in both type mice injected with lipopolysaccharide extremely increased and was significantly higher than those injected with normal saline(p<0.01). Not interleukin-6, but interleukin-10 in ApoM+/+ mice was significantly lower than that in ApoM-/-mice after normal saline injection(p<0.05). Not interleukin-10, but interleukin-6 in ApoM+/+ mice was significantly lower than that in ApoM-/-mice after lipopolysaccharide injection(p<0.01). It is demonstrated that there was interaction between ApoM and lipopolysaccharide while testing their influences on interleukin-6 using two-way analysis of variance(p<0.01), but there was no interaction while testing their influences on interleukin-10.Conclusions: Lipopolysaccharide injection could significantly increase interleukin-10 and interleukin-6 in both ApoM+/+ and ApoM-/- mice, and ApoM could perform anti-inflammatory effect by restraining the elevation of interleukin-6 due to lipopolysaccharide injection.