Effect Of Ex Vivo Oxygenated Blood Continuous Perfusion On Pig Pulmonary Functon And Inflammatory Factors

ObjectiveTo observe the effect of ex vivo oxygenated blood continuous perfusion versus static cold storage the donor lungs on post-transplant pulmonary function and inflammatory factors.MethodAfter being flused with Celsior solution through the pulmonary artery and vein, the Guangxi miniature pig lungs were preserved by ex vivo oxygenated blood continuous perfusion for eight hours(the experimental group, n=6) and static cold storage for eight hours (the control group, n=6) respectively,and then the left lungs were transplanted.Assessed the post-transplant function including the lower left pulmonary oxygen saturation at half of an hour、two hours、four hours and six hours(IR0.5h、IR2h、IR4h、IR6h) after reperfusion and the lung’s wet/dry(W/D) at the time of being perserved before(T0)、after eight hours saved(T1)、three (T2) and six(T3) hours after reperfusion.Observed donor-lung injury by HE staining and the concentration of IL-1β、IL-8 and IL-10 in pulmonary tissue.Results①With the extension of reperfusion time,left lower lung pulmonary vein oxygen saturation gradually declined,and the W/D rised,but the experiment group was significantly higher than the control (P<0.01) in the left lower lung pulmonary vein oxygen saturation at each time.Tl obviously higher than TO in control about W/D,meanwhile,versus the experiment group at T3,W/D significantly higher in the control (P< 0.05).At the same time, the pulmonary edema grading received a statistically significant difference compare the experiment with the control (P<0.05). ②The concentration of IL-1β and IL-8 in both were gradually increased after reperfusion, expecially in the control,which received a higher rises than the experiment group at T3 (P< 0.05).In total, the concentration of IL-1β have significant difference(P< 0.05).The concentration of IL-8 in the experiment group appeared decreased significantly than the control(P< 0.01) at T1、T2 and T3,also the level of IL-8 in two groups appeared differences overall(P< 0.01).At T1, to protect against inflammatory response, the concentration of IL-10 in lung tissues increased,but they did not achieve statistical significance(P>0.05).ConclusionThe results show that the donor lungs are transplanted to receptors after being storaged with ex vivo oxygenated blood continuous perfusion, can obtain a better oxygenation and lighter edema post-transplant. Ex vivo oxygenated blood continuous perfusion may reduce the ischemia-reperfusion injury by inhibiting the release of the IL-1βand IL-8.