Changes And Influencing Factors Of Cholinesterase Activity And Telomere Length In Workers Exposed To Omethoate

ObjectiveThe aims of present study were to learn changes of cholinesterase activity and telomere length in workers exposed to omethoate and to explore the roles of m RNA expressions and genetic polymorphisms of metabolic enzyme genes and telomere binding protein genes. Subjects and methods 1. SubjectsOne hundred and eighty omethoate exposed workers were selected as exposure group, whose working duration was more than eight years, meanwhile one hundred and fifteen unexposed healthy people were chosen as control group. The field exposure data and basic information and blood samples of both groups were collected after obtaining informed consent. 2. MethodsThe whole blood, RBC and plasma cholinesterase activities were detected according to the methods of appendix B in GBZ52-2002. The q PCR technology was applied to measure telomere length. PCR-RFLP and CRS-PCR-RFLP technologies were employed to detect the polymorphisms of telomere binding protein genes and metabolic enzyme genes, telomere binding protein genes involved POT1、TRF1 and TERT, simultaneously metabolic enzyme genes included GSTT1、GSTM1、GSTP1 and CYP2E1. The real-time PCR technology was used to detect the m RNA expressions of POT1, TRF1, TRF2, TIN2, RAP1 and TPP1. 3. Statistical analysisSPSS21.0 software was utilized to analyze experimental data. χ2 test was used to deal with qualitative data. sx ± was employed to describe nomal distrition data and t test or ANOVA was used to compare between groups. M(P25, P75) was used to describe abnormal distribution data and rank sum test was applied to compare between groups. PHASE2.0.2 software was implemented to analyze the effect of diplotype on telomere length. Spearman rank correlation was used to analyse correlation relationship of abnormal distribution data. Multiple linear regression was conducted to analyse the influencing factors of cholinesterase activity and telomere length. Significant level α was at 0.05. Results 1. Cholinesterase activity and telomere lengthThe activities of whole blood, RBC and plasma cholinesterase in exposed group were lower than control group, the difference was statistically significant(P<0.001). The telomere length of exposed group and control group were 1.51(1.10, 2.58)and 0.94(0.76, 1.32)respectively, the difference was statistically significant(Z=-7.864,P<0.001), the telomere length of exposed group was longer than control group. There was a negative relationship between telomere length and whole blood cholinesterase activity(r=-0.182, P=0.002). 2. The m RNA expressions of telomere binding proteinsThe m RNA expressions of POT1、RAP1、TIN2、TPP1 and TRF2 in exposed group were decreased than control group(P<0.05); There were negative correlations between m RNA expressions of POT1 and TIN2 with telomere length(r=-0.145, P=0.031; r=-0.337, P<0.001). 3. The influencing factors of cholinesterase activitySingle factor analysis found that gender, smoking and alcohol consumption had effects on cholinesterase activity in both groups(P<0.05); Multiple factor analysis demonstrated that group(b=-1.436, P<0.001), gender(b= 0.417, P<0.001), alcohol consumption(b= 0.291, P=0.007) and age(b=-0.010, P=0.019) influenced the activity of cholinesterase. However, working duration and metabolic enzyme genetic polymorphisms were not found to have impacts on it. 4. The influencing factors of telomere lengthSingle factor analysis showed that telomere length of(GG+AG) genotype was longer than AA genotype of GSTP1 rs1695 in exposed group(Z=-2.273, P=0.023). Meanwhile, in control group, telomere length of GG genotype was longer than(AA+AG) genotype of TRF1 rs3863242(Z=-2.734,P=0.006), GSTM1 null genotype was longer than non-null genotype(Z=-2.911,P=0.004). Multiple factor analysis revealed that group(b= 0.442, P<0.001), TRF1 rs3863242(b=0.227, P=0.008), TPP1 m RNA(b=0.139, P<0.001), TRF1 m RNA(b=0.109, P=0.005), TIN2 m RNA(b=-0.092, P=0.001), TRF2 m RNA(b=0.027, P=0.011) have impacts on telomere length. Conclusion1. Omethoate occupational exposure induces the decreases in whole blood, RBC and plasma cholinesterase activity and the main influencing factors include gender, age and alcohol consumption, however working duration and metabolic enzyme genetic polymorphisms were not found have impacts on it.2. Omethoate occupational exposure may prolong telomere length and the main influencing factors involve wild genotype of TRF1 rs3863242 and m RNA expressions of telomere binding proteins, the related mechanisms need to be further explored.