Compound Huangqi Yangxin Mixture IS On The Influence Of CX43 Ischemic Arrhythmias Of Rat Myocardial Cells In Site Ser262 And Ser368 Phosphorylation

Objective By means of Compound huangqi yangxin mixture intervention of ischemic arrhythmia in rats myocardial cells Cx43 after 30 minutes in site Ser262 and Ser368 phosphorylation research, explore compound huangqi yangxin mixture clinical mechanisms of ischemic arrhythmias.Methods Application of Compound huangqi yangxin mixture small dose group 7 g/kg/d,14 g/kg/d dose group, high dose group of 28 g/kg/d, Western medicine group of succinic acid metoprolol (9.5 mg/kg/d MMSRT), model group, the control to the amount of 0.9% saline lavage before building on SD rats, after 2 weeks of the left anterior descending coronary artery ligation 30 minutes into ischemic arrhythmia model, recording electrocardiogram (ecg) in ischemia in rats, and then extracted myocardial after 30 mins, marks by ventricular arrhythmia (VA rating), Western blot imprinting technology, HE observed under optical microscope three methods, analysis of each group rats ischemic arrhythmias after 30 minutes the VA rating, myocardial cell Cx43 in site Ser262 and Ser368 phosphorylation levels, and observed the pathological changes of ischemic myocardium under optical microscope.Resultsâ‘ VA rating:Compound huangqi yangxin mixture group(high-dose group, middle-dose group and small dose group)and MMSRT group VA rating were lower than the model group (P< 0.05); The comparison between traditional Chinese medicine group there was no significant difference (P> 0.05).â‘¡Site Ser262 phosphorylation level:Compound huangqi yangxin mixture group (high-dose group, middle-dose group and small dose group) and MMSRT group were higher than that of model group (P<0.05). With high-dose group and MMSRT closer, and compound huangqi yangxin mixture group, high-dose group, the middle-dose group, small dose group there was no statistically significant difference (P> 0.05).â‘¢Site Ser368 phosphorylation level:Compound huangqi yangxin mixture group (high-dose group, middle-dose group and small dose group)and MMSRT group were higher than that of model group (P<0.05). Other than in the low-dose group and there was no statistically significant difference between the middle-dose groups (P>0.05), more than differences between the two groups have statistical significance (P<0.05). Especially in the high-dose group and MMSRT group closer.â‘£HE observed under optical microscope results: By optical microscope CX43 ischemic myocardial cells after 30 min found compound huangqi yangxin mixture group (high-dose group and middle-dose group) and MMSRT group is no of eosinophilic cytoplasm, granular degeneration and coagulation necrosis;And the model group in the early myocardial ischemia is a typical of eosinophilic cytoplasm and granular degeneration and coagulation necrosis; And compound huangqi yangxin mixture (small dose group) also appeared after 30 min of ischemia of eosinophilic cytoplasm and granular degeneration, but does not appear coagulation necrosis.Conclusion Compound huangqi yangxin mixture group has resistance to ventricular arrhythmias. The mechanism of play fight ventricular arrhythmias may be associated with improved site (Ser262 and Ser368) phosphorylation level, promote the function of gap junction newsletter of the play. Optical microscope to observe the ischemic myocardial cells CX43 after 30 mins cardiac pathological changes after HE staining shows that Compound huangqi yangxin mixture group at the same time of play a role of resistance of ventricular arrhythmia and histopathology of ischemic heart disease progression is consistent, indirectly shows that compound huangqi yangxin mixture may be associated by dalaying process of ischemic myocardial cells.