| Objective Establish diabetic rat model,evaluate the impact of glucose and lipid metabolism in animal models by using Hani prescription medicine Qi Hua Granule,to regulate glucose and lipid metabolism in animal of efficacy and safety of a preliminary study,and to explore the mechanism of action, for providing a scientific basis for the study of ethnic medicine of new drugs.Methods Kunming strain mice 40,were randomly divided into 2 groups(Half male and female),Toxicity observed in mice appears,Is calculated Maximum resistance to multiple.Take 56 wistar male rats that were randomly divided into blank group, model control group,Qi Hua Granule prophylactic administration group,Qi Hua Granule low dose group,Qi Hua Granule middle dose group,Qi Hua Granule high dose group,positive control group(The metformin).Experimental group fed with high fat feed besides streptozotocin injected to induce T2 DM rat model,during the experiment observed diets,drinking,weight,blood glucose and other groups of rats.Four weeks after administration,testing blood GLU,TG,TC,HDL-C, LDL-C,Hb A1 c Indicators.Collection liver,kidney,pancreas,to observe the histopathological changes.Results1.Clinical drug safety of the Qi Hua Granule:Acute toxicity test in mice t han is calculated the maximum amount of resistance to the Qi Hua Granule is more than 100 times the clinical dose.Shows that the drug high security.2.The Qi Hua Granule Improve the clinical symptoms of DM rat model:B eginning of the experiment,the weight of rats,food intake,water intake diffe rences were not statistically significant comparable.After the start of the experi ment,T2 DM model rats induced by pre-process(high fat feeding stage) weight on the rise,No obvious change the amount of food and water,Given STZ intrap eritoneal after injection the weight of rats began to decline,increased water inta ke and obvious the symptoms of diabetes.after give the Qi Hua Granule intragas tric a dministration one week weight began to rise,decreased water intake,Mode l control group, the weight has been declining,remain high water intake.Shows that the Qi Hua Granule can effectively improve weight loss of diabetic rats,inc reased water intake and other clinical symptoms.3.The Qi Hua Granule on diabetic rat model fasting blood glucose regulatio n:2 weeks and 4 week after administration of the drug,Compared with the mo del group,each of administered group and positive group in the fasting blood g lucose was significantly lower than the model group,the difference was statistic ally significant(P<0.01).The results Shows that Qi Hua Granule in each dose gr oup fasting blood glucose of a clear regulatory effect,can effectively reduce the fasting blood glucose in diabetic Rat Model.4.Effects of drugs on biochemical indices of each group in the rats:Compared with blank control group,the differences of GLU,TG,TC,HDL-C,LDL-C,Hb A1 c in model control group were statistically significant(P<0.05).Sh ows that this experiment model was successful.4.1 Effects of drugs on rats in the of each group GLU:Compared with t he model group,the differences of GLU each of Qi Hua Granule administered group and positive group were statistically significant(P<0.05).Shows that the Qi Hua Granule can effectively reduce the GLU levels in diabetic rat model.4.2 Effects of drugs on rats in the of each group TG:Compared with the model group,the differences of TG each of Qi Hua Granule administered group and positive group were statistically significant(P<0.05).Shows that the Qi Hua Granule can effectively reduce the TG levels in diabetic rat model.4.3 Effects of drugs on rats in the of each group TC:Compared with the model group,the differences of TC each of Qi Hua Granule administered group and positive group were statistically significant(P<0.05).Shows that the Qi Hua Granule can effectively reduce the TC levels in diabetic rat model.4.4 Effects of drugs on rats in the of each group HDL-C:Compared with t he model group,the differences of HDL-C each of Qi Hua Granule administer ed group and positive group were statistically significant(P<0.05).Shows that t he Qi Hua Granule can effectively reduce the HDL-C levels in diabetic rat mod el.4.5 Effects of drugs on rats in the of each group LDL-C:Compared with the model group,the administration group LDL-C lower than the model group,except for the high dose group, the differences were statistically significant(P<0.05).Shows that,In addition to the high-dose group,the Qi Hua Granule can effect ively reduce the LDL-C levels in diabetic rat model.4.6 Effects of drugs on rats in the of each group Hb A1C%:Compared wi th the model group,each of Hb A1C% each of Qi Hua Granule administered gro up and positive group were statistically significant(P<0.05).Shows that the Qi Hua Granule can effectively reduce the Hb A1C% levels in diabetic rat model.5.The Qi Hua Granule on rats of each group liver,kidney,pancreas histopath ological changes:The control group of rats liver,kidneys,pancreas is normal wit hout obvious pathological changes.Compared with the model group,The Qi Hua Granule administered group,liver of tissue cells Necrosis of blocking less light degree of fatty degeneration;kidneys of low degree of edema,less exudates, less sediments pink;More than the number of islet cells,nuclear condensation and n arrow,light edema and vacuolar degree of degeneration.Shows that the Qi Hua G ranule can effectively improve the pathological organization in the diabetic Rat Model histological changes.Conclusions1.High safety the clinical medication of Qi Hua Granule,and can be widely used in clinical.2.The Qi Hua Granule can effectively improve the clinical symptoms of dia betic rat model,effective mitigation pathological signs of diabetic rats,improve t he symptoms of diabetes.3.The Qi Hua Granule can improve glucose and lipid metabolism in diabeti c Rat Model GLU,TG,TC,HDL-C,LDL-C,Hb A1 c indexes of glucose and lipid metabolism in diabetic rats induced abnormalities are significantly improved.4.The Qi Hua Granule for pathological changes of diabetic rats histology a nd treatment can delay,impede the restoration of function and progression of th e disease effect significantly. |
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