| Objective:Reproduce the model of nonalcoholic fatty liver disease in rats. Analyze the relationship between nonalcoholic fatty liver disease and oxidative stress reaction. Discuss the prevention and cure function of Xuesaitong in the model of nonalcoholic fatty liver disease in rats.Methods:1. Animals groups:Weigh 40 male clean SD rats, according to the weight and random number table,the rats were randomly divided into four groups, each group has 10 rats.The normal group(10),model group(10), vitamin C group(10),Xuesaitong group(10).2. Model preparation:Normal group used ordinary feed; the rest three groups used high fat diet.3. Medication:From 1st week to 12th weekend, normal group and model group were conducted with 0.9% saline to fill the stomach. Vitamin C and Xuesaitong group were conducted with vitamin C diluent and Xuesaitong diluent to fill the stomach respectively.4. Measuring index:Weighed each rat once a week; On 12th weekend put the rats to death and inspected each rat liver function (ALT, AST) and serum lipid (serum TC, TG). Make paraffin section of liver tissue and conduct HE staining. Use the enzyme linked immunosorbent assay method (ELISA) to detect ATP content in each rat liver tissue. Use the xanthine oxidase method to detect SOD content in each rat liver tissue; Use Rt-pcr (realtime PCR) to detect transforming growth factor beta 1 (TGF-《β1) expression quantity of each rat liver tissue.Results:1. The weight of normal group were normal, light color, relatively lively. The weight of model group were increased more obviously than the normal group, the difference was statistically significant (p< 0.05).2. Pathology:(1) HE staining shows that the liver tissue of normal liver cells are uniform, liver cell nucleus are circular, hepatic cords arrange radially; Model group has a large number of fat vacuoles, cytoplasmic loosening, hepatic cords arrange in disorder; Xuesaitong group also could see fat vacuoles, but smaller than model group.3. Serum biochemical:compared with normal group, the serum ALT, AST increased in model group, the difference was statistically significant (p< 0.05); Serum TC, TG increased in model group, the difference was statistically significant (p < 0.05); Compared with model group, the content of serum ALT, AST of Xuesaitong group were lower than model group, the difference was statistically significant (p< 0.05); The serum TC, TG were lower than model group, the difference was statistically significant (p< 0.05).4. Liver tissue SOD content:compared with normal group, model group rats liver tissue SOD levels were significantly lower, the difference was statistically significant (p< 0.05); Xuesaitong group rats liver tissue SOD levels significantly increased, the difference was statistically significant (p< 0.05).5. ATP content in liver tissue:compared with normal group, model group rats liver tissue ATP content in liver tissue were significantly lower, the difference was statistically significant (p< 0.01);Compared with model group, the Xuesaitong group rats liver tissue ATP content in liver tissue increased, the difference was statistically significant (p< 0.01).6. Liver tissue expression of TGF-Pr. compared with normal group, model group liver tissue expression of TGF-β1 quantity increased, the difference was statistically significant (p< 0.05); Compared with model group, the Xuesaitong group liver tissue expression of TGF-β1 quantity decreased, the difference was statistically significant (p< 0.05).Conclusion:1. High fat diet can successfully reproduce rat model of nonalcoholic fatty liver disease(formula:88% Ordinary feed+10% lard+2% cholesterol).2. SOD content reduced in rat model of nonalcoholic fatty liver disease, which indicated that in the development of nonalcoholic fatty liver disease could have oxidative stress.3. Model group ATP content in liver tissue reduced, which indicated that in the development of nonalcoholic fatty liver disease could have mitochondrial damage. Xuesaitong group ATP content increased, which can improve the mitochondrial damage.4. Model group liver tissue expression of TGF-β1 quantity increased, which indicated that TGF-β1 might be involved in oxidative stress which could damage to the liver, it also need to be further proved.5. Xuesaitong can reduce the degree of fatty degeneration in nonalcoholic fatty liver model rats, improve the oxidative stress reaction and lipid peroxidation, and reduce the content of TGF-beta 1 for the prevention and treatment of nonalcoholic fatty liver disease. |
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