Changes Of T Lymphocyte Subsets In Peripheral Blood After Renal Transplantation In Rats

Objective: To analyze the relationship between the changes of T lymphocyte subsets and acute rejection(AR) after renal transplantation in rats. Methods: Control group consisted of 15 normal SD male rats. Sham-operation group consisted of 15 SD rats. 16 SD rats receiving SD syngenegic renal grafts were as non-AR group. 16 SD rats receiving allogenegic renal grafts from 16 Wistar rats were as AR group. T lymphocyte subsets and kidney function were detected. The degree of acute histopathologic rejection was semiquantitatively scored. Results: The levels of serum creatinine((115.35±30.09) μmol/L), urea nitrogen((19.80±10.71) mmol/L) and AR semiquantitative score(3(3, 3)) were significantly higher by day 7 after operation in AR group than those in control group((17.80±4.45) μmol/L,(4.97±0.49) mmol/L, 0(0, 0)), sham-operation group((47.06±4.28) μmol/L,(5.75±1.06) mmol/L, 0(0, 0)) and non-AR group((55.31±8.48) μmol/L,(8.25±1.57) mmol/L, 0(0, 1))(P<0.01). The proportion of CD4+ in T cells and the CD4+/CD8+ ratio were significantly higher in AR group((27.98±6.07)%, 2.13±0.66) than those in non-AR group((8.23±2.63)%, 0.20±0.06), sham operation group((9.84±2.87)%, 0.22±0.10) and control group((8.94±2.44)%, 0.20±0.07)(P<0.01). The proportion of CD8+ in T cell was significantly lower in AR group((13.85±3.74)%) by day 7 day after operation than that in non-AR group((43.08±6.26)%), sham operation group((46.67±6.94)%) and control group((43.69±9.82)%)(P<0.01). Conclusion: The detection of T lymphoyte subsets has significant value to evaluating the immunity of recipients undergoing renal transplantation, contributing to the early diagnosis and treatment of AR.