In Vitro Evaluation Of The Effect Of CGF And PRF On Promoting Vascularization

Background:Dental implant are most innovative and superior treatment indentistry.Specially, The application of Guide Tissue Regeneration(GTR) andGuided Bone Regeneration(GBR) has lead to the development of new material.Tissue regeneration therapies are great importance to the whole scientificcommunity. To attempts to reconstructure a complex structure have ledresearcher and clinicians to very promise results. Choukroun’s platelet-richfibrin(PRF) is an autologous leucocyte- and platelet-rich fibrin material. PRFhas been used in different clinical application as well as engineering tissue.Nevertheless Concentrated Growth factor(CGF) seems to have a betterregenerative capacity and versatility.The potential of these preparations is thefibrin network that contains platelets, leukocytes, and growth factors andprovide a matrix for cell migration.Objectives: To make further understanding Of Platelet rich fibrin(PRF) andConcentrated Growth Factor(CGF) Biological characteristic and In vitro Valuesin Vascularization effects through qualification of Human Umbilical Endothelialcell proliferation and differentiation. It will offer more supportive evidences ofthe role PRF and CGF in Bone regeneration process.Material and Study Design1. Whole blood samples from healthy participant were drawn to generate PRFand CGF.2. PRF- and CGF- preparations added for 5 days with difference of density.3. Human umbilical endothelial vein cells was used for the cell culture. Cell linewas separated and cultivated from human umbilical cord. We used thirdgeneration of cell line to do In vitro research.4. Cell proliferation was determined by Cell counting kit- 8 Assay(CCK8).5. The drawn medium was pooled and the quantities of growth factors( VEGF-Vascular endothelial growth factor, TGF-beta1 transforming growth factorbeta1) growth factor isomer.TGF-beta1 and VEGF of all samples were quantified using a commerciallyavailable ELISA( enzyme-linked immunosorbent assay) kit.Statistical AnalysisSPSS 19.0 was used in statistical analysis. All data are expressed asmean+_standard deviation.P-value less than 0.05 was considered statisticallysignificant.Result1. Cell proliferation CCK8 counting2. CGF and PRF promote HUVE cells proliferation.3. The effect of CGF in HUVE cells proliferation significantly higher than PRF.4. ELISA assay results1) For vascular endothelial growth factor(VEGF) and transforming growthfactor beta 1(TGF-b1), the values obtain in CGF samples were higher PRFsamples.2) Both VEGF and TGF-b1 levels in PRF and CGF were decreasing day by dayin cell culture design.Conclusion1. Platelet rich fibrin(PRF) and concentrated growth factor(CGF) significantlypromote human umbilical vein endothelial cell(HUVEC) proliferation anddifferentiation.2. CGF membrane have higher amount of growth factor VEGF to compare withPRF membrane. VEGF and TGF beta1 have a role in vascularization of HUVEcells In vitro.