| Objective:observe the expression of transforming growth factor-beta 1(TGF-β1), connective tissue growth factor(CTGF), nuclear transcription factor(NF-kappa Bp65) and tumor necrosis factor(TNF-α) in the kidney in diabetic rats, discuss the inhibitory effect of20(S)- ginsenoside Rg3(hereinafter referred to as Rg3) on diabetic rats renal fibrosis and inflammation and apoptosis.Methods:his study established diabetic rats model by high sugar, high fat diet combined with intraperitoneal injection of streptozotocin(STZ Streptozotocin); Using 20(S)-ginsenoside Rg3 interference and detect the biochemical indicators of the experimental rats. Through the periodic acid Schiff(PAS) staining detect the change of glomerular basement membrane and mesangial matrix; Immunohistochemical staining was to detect the expression of fibrosis factors in transforming growth factor beta 1(TGF- beta 1) and connective tissue growth factor(CTGF),Tumor Necrosis Factor-α(TNF alpha) and nuclear transcription factors- k B(NF- k B)in the rat kidney tissue. Through the TUNEL kit to detect glomerular and renal tubular cell apoptosis.Result:1. Compared with the normal control rats, diabetic rats significantly reduced weight(P < 0.05), whereas 20(S)- Rg3 treatment group significantly increased the weight of the rats(P < 0.05).2. The urine protein of diabetes group rats obviously higher than normal control group(P < 0.05) and 20(S)- Rg3 treatment group decreased in the diabetic group(P< 0.05); Fasting blood sugar, Cr, TC, and TG of diabetes group rats were significantly higher than that of normal control group(P < 0.05), however, fasting blood sugar, Cr,TC, and TG of 20(S)- Rg3 treatment group rats were lower than that of diabetes group, but there was no statistically significant difference(P > 0.05).3. Through the PAS staining,we observed the increased glomerular volume of the diabetes mellitus group, narrow cystic cavity crevice, glomerular mesangial matrix and mesangial cell diffuse mild hyperplasia, and 20(S)- Rg3 treatment group only mesangial matrix and segmental mild hyperplasia of mesangial cells.4. TGF- beta 1, CTGF, NF-kappa B65 and TNF alpha factor expressed in kidney in rats in each group.Diabetes group of each protein expression in kidney tissue was normal control group increased significantly(P < 0.05); Compared with diabetes group, 20(S)- the protein expression of Rg3 treatment group were significantly lower(P < 0.05).5. Diabetes group and 20(S)- Rg3 renal tubular epithelial cell apoptosis in the treatment group were significantly greater than the control group(P < 0.05);Compared with diabetes group, 20(S)- Rg3 apoptotic cells in the treatment group decreased significantly(P < 0.05).Conclusion:1. 20(S)- ginsenosides Rg3 can improve diabetic rats body weight, can reduce diabetic rats urinary protein.2. 20(S)- ginsenosides Rg3 inhibits the apoptosis of diabetic rats renal tubular epithelial cells.3. 20(S)- ginsenosides Rg3 can reduce the expression of kidney inflammation related factor TNF alpha, the NF-kappa Bp65 and fibrosis related factor CTGF, TGF-beta 1 in diabetic rats.4. 20(S)- ginsenosides Rg3 plays a protective role in diabetic rats kidney inflammation and fibrosis. |
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