Secondary Metabolites Of Three Marine-derived Endophytic Fungi

In order to find new leading compounds with bioactivities from the secondary metabolites of marine-derived fungi, more than ten strains were isolated from various marine environments. Through the analysis of their capacity of producing compounds in pre-experiments, Aspergillus sp. Z-3, from the gut of Ligia exotica, Simplicillium sp. AF3-2 and an unidentified fungus QD1, from red alga, were chosen to investigate the secondary metabolites of them by large-scalely culture.The ethyl acetate extract of the broth and methanol extract of fermented mycelia were separated by modern chromatographic methods, such as silica gel column chromatography, Sephadex LH-20, TLC and preparative reversed-phase HPLC. The structures of the compounds isolated were elucidated on the basis of their spectroscopic data analysis.Eleven compounds were obtained from the fungal fermentation, and their structures were elucidated as:diorcinol (1), regeione (2), WIN 64821 (3), and two novel compounds 4 and 5 from Z-3, antifungal agents verlamelin A (6) and griseofulvin (7) from AF3-2, altemariol (8), altemariol methyl ester (9), altenuene (10), 5’-methoxy-6-methyl-biphenyl-3,4,3’-triol (11) from QD1.Cytotoxicity of verlamelin A against the PC3 cell line and SW620 cell line was assayed using the MTT method. However, verlamelin A showed almost no activity.