| Objective : Through the observation of the expression levels of superoxide dismutase(SOD),reactive oxygen species(ROS) and the pathological changes of lung tissue in COPD model rats exposed to cigarette smoke,which is under intervention of Modified Decoction of Liujunzi, the research aimed to explore oxidation-ant oxidation regulating mechanism of the decoction of medified Liujunzi to rat models of COPD.MethodsExperiment 1: 40 SD rats, according to the random number table, were randomly divided into four groups, with 10 rats in each group:blank group(A), smoking 8 weeks group(B), smoking 12 weeks group(C), smoking 16 weeks group(D).With the method of fumigation, rat models of COPD were prepared.16 cigarettes each time(sustained about 2hours),2 times a day(in the morning and afternoon).After the modeling, intraregional injection of pentobarbital sodium anesthesia, remove the lung tissue in rats, made HE staining, and then observe pathological changes of lung tissues in rats under the light microscope. SOD and ROS were both quantitatively analyzed in peripheral blood of rats by avidinbiotincomplex-Enzyme-Linked Immuno Sorbent Assay(ABC-ELISA).Experiment 2: According to the experimental 1 results,a total of 60 SD rats were randomly divided into blank group(10 SD rats) and smoking group(50 SD rats).The blank group was fed for 16 weeks normally,and the smoking group was smoked for16 weeks.Smoking methods are the same as the experiment 1.After the modeling, A total of smoking group,according to the random mumber table, were randomly divided into model group, Acetylcysteine Effervescent Tablets group(Fulushi Group), Modified Decoction of Liujunzi( high dose group), middle dose group, and low dose group.10 rats in each group.Blank group and model group treated with physiological saline by gavage for 4weeks, the other group rats were given corresponding drug intervention for 4weeks.After drug intervention,intraperitoneal injection of pentobarbital sodium anesthesia,remove the lung tissue in rats,made HE staining,and then observe pathological changes of lung tissues in rats under the light microscope.SOD and ROS were both quantitatively analysed by ABC-ELISA.ResultsExperimental 11.The symptoms of smoked rats at different time interval:as the smoke time extended, the uncomfortable symptoms of rat gradually increased.2.The pathological change of smoked rats lung tissue at different time interval:with the smoking time prolonged, the degree of rat lung tissue injury was gradually aggravated.3.The level of SOD in peripheral blood of rats at different time stage of smoke:Group B(154.3±7.26),group C(129.3±5.10),group D(110.3±6.77)compared with group A(179.2±2.86), the difference was statistically significant(P<0.05, P<0.05, P<0.05).Group C, group D compared with group B, the difference is statistically significant(P<0.05, P< 0.05). The difference between Group C and group D is statistically significant(P<0.05).4.The level of ROS in peripheral blood of rats at different time stage of smoke:Group B(359.8±12.79),group C(399.6±11.68),group D(428.6±13.75)compared with group A(329.1±6.77), the difference was statistically significant(P<0.05, P<0.05, P<0.05).Group C, group D compared with group B, the difference is statistically significant(P<0.05, P< 0.05). The difference between Group C and group D is statistically significant(P<0.05).Experimental 21.Symptoms of rats:model group compared with blank group rats, appear different degree of unwell symptoms.Compared with model group,the discomfort symptoms of Fulushi group, high dose group and the middle dose group rats significantly decreased. The discomfort symptoms of low dose group rats have no obvious improvement.2.The pathological changes of the lung tissue in rats: compared with the blank group, model control group can been seen bronchial cilia falling off,vascular dilatation and congestion, and alveolar septum widened significantly. A large number of lymphocytes,a small amount of phagocyte and neutrophil infiltration in peripheral blood vessels and bronchi.Alveolar walls broke fused and formed alveoli emphysema.Compared with the model group,Fulushi group,high dose group and the middle dose group rat lung tissue pathological damage reduced significantly,low dose group had no obvious improvement of pathological damage.3.The comparison of SOD in the peripheral blood in different groups(1)Model group(108.30±2.45) compared with blank group(178.50±2.64),the difference was statistically significant(P<0.05).(2)Fulushi Group(175.00±1.15),high dose group(174.90±1.20),middle dose group(174.80±2.66)compared with model group,the difference was statistically significant(P<0.05,P<0.05,P<0.05).The difference of low dose group(112.80±2.39)and model group was not statistically significance(P>0.05).(3)The difference between high dose group, middle dose group and Fulushi group was not statistically significant(P>0.05,P>0.05).The difference between Fulushi group and low dose group was statistically significant(P<0.05).(4)High dose group compared with the middle dose group, the difference had no statistical significance(P>0.05).The difference between high dose group and low dose group was statistically significance(P<0.05).4.The comparison of ROS in the peripheral blood in different groups(1)Model group(444.59±3.38) compared with blank group(302.41±2.75),the difference was statistically significant(P<0.05).(2)Fulushi Group(303.14±2.61),high dose group(304.33±2.98),middle dose group(302.93±2.88)compared with model group,the difference was statistically significant(P<0.05,P<0.05,P<0.05).The difference between low dose group(444.54±3.28) and model group was not statistically significance(P>0.05).(3)The difference between high dose group, middle dose group and Fulushi group was not statistically significant(P>0.05,P>0.05).The difference between Fulushi group and low dose group was statistically significant(P<0.05).(4)High dose group compared with the middle dose group, the difference had no statistical significance(P>0.05).The difference between high dose group and low dose group was statistically significance(P<0.05).Conclusion1.With the smoking time extended, the level of SOD in rat peripheral blood decreased,the level of ROS increased.Unwell symptoms and the rat lung injury degree gradually increased.The change of model rats for 16 weeks of smoking was the most typical.2.Modified Decoction of Liujunzi have antioxidant properties.High dose group and middle dose group can significantly improve the level of SOD which in peripheral blood serum of COPD rat model,and lowered the level of ROS.3.Modified Decoction of Liujunzi can reduce lung inflammation of cigarette smoke exposure rats models,and delay the process of COPD. |
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