| Objective: By studying the correlation analysis on the volatile oil form Ferula ferulaeoides from difefrent habitats with its genetic diversity and ecological factor, to screen the dominant factors, which may help to guarantingee the clinical validity, and to provide a scientific basis for revealing the suitable growth mechanism, and for the evaluation of the resources and the quality of F. ferulaeoides. Methods:(1) The content of volatile oil of Ferula ferulaeoides from different habitats were extracted by GC-MS and analysed by the method of One-way ANOVA.(2) The genetic diversity of 176 samples from 8 counties in the province was studied by using ISSR molecular markers technique. The effect of genetic diversity on the content of volatile oil was analyzed by Grey Relational Analysis(GRA).(3) Determination of soil nutrients and p H values in different habitats and using the methods such as One-way ANOVA, correlation analysis, partial least squares(PLS) and Grey Relational Analysis(GRA) to analyze the soil factors which effect on the volatile oil content of F. ferulaeoides much more.(4) The influence of meteorological factors on the content of volatile oil form F. ferulaeoides was analyzed by rank correlation and GRA.(5) The influence of Genetic, ecological factors on the content of volatile oil form F. ferulaeoides was analyzed by GRA. Results:(1) There were significant differences in the contents of volatile oil form F. ferulaeoides in different regions.(2) The ISSR analysis of the F. ferulaeoides samples was carried out by using the 15 primers, The highest level of genetic diversity among the 8 populations was Yu Min, and the lowest population was Qinghe. By calculation the genetic diversity of Nei’s diversity index, Shannon’s information index and polymorphism site index percentage of F. ferulaeoides populations, the result showed a strong correlation to the volatile oil, especially to Ginsenol, Camphor, γ-Eudesmol and total volatile oil.(3) The soil factors from different habitats were significantly different. The total content of F. ferulaeoides’ 12 volatile components and soil p H value were significantly negative correlation(P < 0.05), the content of guaiol showed a significant negative correlation with the available K(P < 0.05), the α-pinene was significantly positive correlation with the available N,K(P < 0.01), the content of camphene and d-limonene showed significant positive correlation with the available K(P < 0.05), bulnesol and total phosphorus showed significant positive correlation(P < 0.05), and β-Famesene and available phosphorus were significantly negative correlation(P < 0.05). By the analysis of PLS, the soil p H value is important to the volatile oil formthe F. ferulaeoides, such as the content of available K. GRA showed that the p H value, natural water content and available K in the soil were large correlation to the volatile oil formthe F. ferulaeoides. Conclusion:(1) The contents of volatile oil of F. ferulaeoides from different habitats were significantly different.(2) The Nei’s diversity index among the three factors have a greater impact on the volatile composition and content of F. ferulaeoide.(3) The soil factors of different habitats were significantly different. The correlation analysis, PLS analysis and GRA showed that the soil p H value, the content of available K, and natural water content were the main factors that could influence the content of volatile oil form F. ferulaeoides.(4) Rank correlation and grey correlation analysis showed that the dominant factor in the meteorological factors is the average annual relative humidity and the average annual sunshine hours.(5) Comprehensive the correlation analysis on the effective components of the F. ferulaeoide and the genetic, soil and meteorological factors, it show that soil factors are the dominant factors affecting the quality of the F. ferulaeoide, and it has important significance for the accumulation of volatile oil form F. ferulaeoide. |
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