Human Body Burden Evalution And Detection Technology Of PCDD/Fs And Dl-PCB In China

Polychlorinated dibenzo-p-dioxins and dibenzofurans(PCDD/Fs)and dioxin like polychlorinated biphenyl(dl-PCBs) are groups of organochlorine compounds that belong to a list originally comprised of 12 persistent organic pollutants(POPs) that are regulated under the Stockholm Convention on POPs. They are ubiquitous, bioaccumulative, toxic and persistent. They can penetrate into the ecological environment and be long-term presence.This study aimed to establish a novel robust method for simultaneous determination of PCDD/Fs and dl-PCBs in serum and human milk. At the same time, we carried out a study to evaluate the human body burden through the breast-monitoring, obtain the internal exposure about body burden.1. Levels of PCDD/Fs and dl-PCBs in breast milkAccording to the guideline from WHO, human milk were collected in 16 provinces and countries, in each sampling site, we have a urban site and two rural sites, collected 50 samples in urban site and 30 samples in rural sites, then the samples were pooled, respectively. PCDD/Fs and dl-PCBs were dectected in human milk samples, the relevance ratio were all above 94%. The level of PCDD/Fs and dl-PCBs from urban site and rural site was 141.6±33.8 pg/g fat and 2751.1±100.4 pg/g fat, 121.4±26.8 pg/g fat and 2554.0±331.3 pg/g fat, respectively. The average level of total TEQ in human milk from urban site and rural site was 6.9±3.1 pg TEQ/g fat and 6.5±2.5 pg TEQ/g fat. Generally speaking, the situation of high content were found in industrialization cities. The most abundant congeners of PCDD/Fs and dl-PCBs were 1,2,3,4,6,7,8-Hp C D,OCDD,PCB-105,PCB-118 and PCB-156.2. Investigation of PCCD/Fs and dl-PCBs in serum and human milk by Isotope Dilution-GCx GC-gas chromatography- tandem mass spectrometryA method based on GCx GC-gas chromatography-tandem masspectrometry(GCx GC-MS/MS) was developed for the determination of PCDD/Fs and dl-PCBs. Serum and human milk were extracted by accelerated solvent extraction and purified by fluid management system.Then analysis the target congeners by GCx GC-gas chromatography – tandem mass spectrometry with DB-5MS and BPX-50,the calibration curves of seventeenth target compounds showed good linearity within R2> 0.99 and the adding standard recovery were between 80%~120%. The estimated value of standard reference materials(SRM1954 and SRM1958) were reliably. With high selectivity and sensitivity, the method was can apply to analysis the PCDD/Fs in serum and human milk.