The Hormone Effect And Its Determination Of Total Suger Of The Wall-broken Pius Massoniana Pollen

Pinus massoniana pollen health products was known as the "green gold" in health food, pollen of Pinus massoniana pollen when compared to other classes,with more types of nutrients, and matching the most reasonable. Pollen of Pinus massoniana natural pollen contains protein, polysaccharides, vitamins, amino acids, trace elements, more than 200 kinds of nutrients. Masson pine pollen can improve immunity, anti-aging, anti-fatigue, beauty beauty, such as health care, are waiting in health food. We found that pollen of Pinus massoniana to hormone-like effect of Pinus massoniana pollen and are very high in sugar.Aqueous extract of Masson pine pollen in Shandong as raw materials, by phenol-sulfuric acid method by UV-Spectrophotometric method for the determination of total sugar in water extract of pine pollen content and precision, repeatability, stability and recovery aspects of the study. Results showed that broken pine pollen water mention real in the contains rich of total sugar, content for 60.22%, in 0.0025-0.0125mg/m L of range within total sugar of concentration and sucking photometric rendering good of linear relationship(r = 0.9991, n= 5), the method operation simple, fast, accuracy, and repeat sex and stability better, can for broken pine pollen water mention real in the total sugar content determination, and for medicine and natural product in the total sugar of content determination provides reference. Ethanol extract of pine pollen can be replaced for two days, is colorless crystallization, standing time Crystal can be increased and more. Crystal h filter, the filtrate continues to rest for placing, you can still continue to produce h, repeat the steps above, will be repeatedly washed with ethanol crystallization(Crystal insoluble in alcohol, easily soluble in water) identification of its structure by NMR and single crystal diffraction for disaccharides. Under HNMR, CNMR, H-H cosy, HSQC and HSBC to determine compound h for sucrose. We further on pine pollen extract sucrose crystallization conditions were investigated in this preliminary study on broken Masson pine pollen extract and broken Masson pine pollen extract water extracting and alcohol precipitation, n-butanol extraction methods are a mix of hormonal activity. In addition, our broken Masson pine pollen extract by UV-Spectrophotometric method for the determination of total sugar content in broken Masson pine pollen at the same time, we studied from the broken Masson pine pollen in the ethanol extract of optimal conditions of formation of sucrose crystallization. Considering the effect on the yield of crystalline condition from the extraction time, temperature, concentration of reagents into consideration, results show that optimal extraction time of 3 hours; the optimum temperature was 40 degrees; most optimum extraction reagent is 80%. Experimental investigation and optimization of sucrose crystallization conditions in pine pollen, pine pollen in the sugar Research Foundation in the late... Broken Masson pine pollen extract 85% alcohol, after removal of precipitation concentration tested in concentrated mixtures have a hormone-like effect, proved by detection broken Masson pine pollen extract has estrogen-like effects, male hormone-like effects。...