Association Between Formation Of Coronary Collateral Circulation And Plasma MicroRNA-210in Patients With Coronary Artery Disease

Objective:The aim of this experiment is to investigate the relationship between the formation of coronary collateral circulation and the expression of microRNA-210 in plasma in coronary artery disease patients.Method:In this study, a total of 80 coronary artery disease patients with ≥90% stenosis in one epicardial coronary artery were enrolled. These patients were divided into two groups according to Rentrop Grades: good CCC group(patients with grape 2 or 3 collateral circulation)(n=24), poor CCC group(patients with grape 0 or 1 collateral circulation)(n=46).Select healthy people(n=13) with no stenosis as normal control. The expression of microRNA-210 in plasma and concentration of VEGF was measured by Real-time PCR and ELISA, respectively. The severity of stenosis in coronary artery was evaluated by Gensini score system.The data was analyzed by SPSS 20.0.Results:The Gensini score was significantly higher in good CCC group than that in poor CCC group(112.77±47.26 vs 60.78±32.52,P<0.05. The HDL-C was significantly lower in good CCC group than that in poor CCC group(1.04±0.20 mmol / L vs 1.19±0.26 mmol /L,P<0.05). The expression of microRNA-210 in plasma(0.85±0.46 vs 1.14±0.52,P<0.05)and concentration of VEGF[(2689.57 ±880.46)ng/L vs(3232.078±820.07)ng/L,P<0.05) ] were significantly lower in coronary heart disease patients, compared with normal control groups. The expression of microRNA-210 in plasma( 0.70 ± 0.42 vs0.93±0.47,P<0.05)was significantly lower in good CCC group in comparison with poor CCC group. The VEGF level in good CCC group was lower than that in poor CCC group,but there was no statistical significance [(2425.31±761.57)ng/L vs(2827.44±914.08)ng/L,P=0.069]. Pearson correlation analysis showed that there was no correlation between microRNA-210(r=0.165, p=0.173) or VEGF(r=0.099, p=0.416) and Gensini scores in coronary heart disease patients. However, there was positive correlation between microRNA-210(r=0.630, P=0.01) or VEGF(r=0.613, P=0.01) and Gensini scores in good CCC groups. Logistic regression analysis showed that micro RNA-210(OR = 0.022, P =0.010) and Gensini score(OR = 1.069, P = 0.001) were independent predictors ofcollateral formation.Conclusion:The trends of the expression of microRNA-210 and VEGF in coronary heart disease patients were the same, and we can conclude that microRNA–210 take part in the regulation of VEGF in the formation of coronary collateral circulation.