| Background:Pituitary adenoma is one of the most common intracranial tumors. The incidence rate in population increased year by year with the development of social economy, the improvement of the diagnosis level and the treatment technology. Although most pituitary adenomas are benign, some of them show invasive growth, aggressing the cavernous sinus, the internal carotid artery, the optic nerve, and the skull base bone, etc. Such tumors are clinically referred to as invasive pituitary adenomas(IPA). Since there is no uniformed standard of the diagnosis and treatment, together with the characteristic of invasive growth, it is difficult to be completely removed by operation. Even after a successful operation, they still have a high percentage of relapse. In view of the facts mentioned above, the treatment of IPA has been a difficult problem in the field of neurosurgery. Nevertheless, recent researches suggest that the non-coding RNA H19 shows an increased expression in a variety of malignant tumors, such as ovarian cancer, bladder cancer, etc., and plays an important role in their growth, differentiation and metastasis. However, the expression of H19 in pituitary adenomas and its relationship with IPA have not yet been studied. Objective:This paper is to analyze the expression of the long non-coding RNA H19 in pituitary adenoma, and to investigate the correlation between the long non-coding RNA H19 and the invasiveness of pituitary adenomas, hoping it could provide some theoretical and experimental basis for further studies of the function of H19 in pituitary adenomas. In addition to these, it also attempts to explore the potential therapeutic value of H19 in the treatment of IPA. Methods :1. Collecting the clinical specimens’ data and statistics. According to the inclusion criteria, we have collected 50 clinical specimens of pituitary adenomas from October 2014 to June 2015 in the Department of Neurosurgical Oncology, The First Bethune Hospital of Jilin University. And based on the Knosp’s five-level taxonomy in pituitary adenoma, the specimens were divided into two groups, 27 cases of invasive pituitary adenoma is based on the level 3~4, and 23 cases of noninvasive pituitary adenomas is based on the level 0~2.2. Extraction and purification of Total RNA from the collected clinical specimens, and then the inspection to Total RNA. All qualified samples went through q RT-PCR, and drew the amplification curve and the melting curve, thus receiving the quantitative data on genes in each specimen.3. Analysis of data:(1) the 2-△ △ CT result was got by calculating the quantitative data of the obtained genes, then the statistics of the genetic expressions between the two groups of samples were compared.(2) The software of SPSS21.0 was applied to the data analysis, and the Mann-Whitney U test which tests two independent samples was used to analyze the different levels of H19 expression in both the invasive and non-invasive groups of pituitary adenomas, thus studying the correlation between the expression level of H19 and the invasiveness of pituitary adenoma. Result:1. The results of the HE staining test in the collected specimens all conformed to the requirement.2. 27 Total RNA samples were got in the experimental group, then after the Total RNA quality inspection, 7 samples were unqualified and 20 samples were qualified. Meanwhile, 23 Total RNA samples were extracted in the control group, then after the Total RNA quality inspection, 6 samples were unqualified and 17 samples were qualified.3. All qualified samples were adapted to the q RT-PCR, receiving the corresponding data which was analyzed by the software of SPSS 21.0. After the U test and some investigation, we found that the expression level of H19 was significantly higher in the invasive pituitary adenoma group than in the non-invasive group(P< 0.05, P = 0.0033). Conclusion:The H19 expression can be detected in both invasive and noninvasive pituitary adenomas, but the level is significantly higher in the invasive pituitary adenomas than in the noninvasive pituitary adenomas. The enhancement of H19 expression may be related to the excessive proliferation of the pituitary adenoma cells and the invasiveness of the pituitary adenoma. Therefore, the H19 may become a marker to predict the bad prognosis of the IPA and its relapse after the surgical operation. Furthermore, the related research of H19 help people to have a more thorough and profound understanding of the invasive tumor’s occurrence and the regular pattern of its development. In conclusion, it can provide us new criteria in the evaluation of the diagnosis and prognosis of the invasive tumors, and also give new ideas to the targeted gene therapy. |
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