| Alcoholic liver disease is a healthy liver damage caused by alcohol consumption, alcohol consumption country is a big country, with social progress and the development of civilization, following the addition of alcohol abuse has become the virus that causes liver damage one of the biggest causes of liver damage caused by alcohol gradually attracted people’s attention.Studies have found that patients with alcoholic liver disease, abnormal immune responses and abnormal cell gene regulation probe into mitochondria swelling, functional decline, and even mitochondrial DNA replication is suppressed, the number of mitochondria and mitochondrial biogenesis decreased significantly, leading to apoptosis happened.Mitochondrion has played a pivotal role in the development and progression of alcoholic liver disease, we should look more on mitochondrial mechanism of the damage of alcohol among the mitochondria as the focus of the study.Objective:After one hand Hovenia silymarin compatibility protection alcoholic liver injury in rat liver mitochondria to research, investigate their compatibility could have a "1+1> 2" synergy, compatibility with modern science theory Study data.The second clinical syndrome diagnosis and treatment of alcoholic liver injury, Herbal Prescription provide scientific guidance value, in order to develop hospital preparation and follow-up studies provide experimental data.Methods:This paper uses ethanol feeding methods replication model of alcoholic liver injury were observed single herb milk thistle and single flavor Hovenia compatibility group and its influence on alcoholic liver injury in rats with liver indicators.In biology, enzyme tissue morphology as the main technical and organizational aspects to elaborate comparison unit Hovenia and silymarin group and its compatibility Protective effects and mechanism of action of alcoholic liver injury in mitochondria.Indicators and Results:1.On alcoholic liver injury in rats with liver weight index:the model group and control group comparison, significantly increased liver index (P <0.01), each administration group silymarin group, orange dulcis group, and 1:2 rats liver index was significantly lower than the model group and has a significant difference,2:1 in liver compatibility index lower than other groups, and has a significant difference.2.Transaminase activity of serum Alcoholic Liver Injury:levels of serum alanine aminotransferase(ALT)and aspartate (AST) were significantly higher than the control group, each treatment group compared with the model group were lower model group and has a significant difference. However, ALT, AST value among the treatment group compared to the group which did not find the effect of outstanding.3.Influence of alcohol pathological changes in liver tissue of liver injury: a single flavor silymarin group, a single flavor Hovenia group and combination group could improve steatosis and inflammatory changes in alcoholic liver injury in rat liver tissue, reduce the damage of alcohol on the liver cells. Wherein the milk thistle and orange dulcis Compatibility 2:1 silymarin group and orange dulcis Compatibility 1:1 group significantly better than other groups and has a significant difference.4.Detection of alcohol-induced liver injury in rat liver mitochondria in liver cell-permeable open channel operation(PTP):the model group rat liver tissue mitochondrial PTP opening, leading to mitochondrial swelling,540nm absorbance at A540 reduction (P<0.01), significantly lower than the control group, each treatment group compared with the model group were significantly lower than the control group and statistical differences. Wherein the milk thistle and orange dulcis Compatibility 1:1 silymarin group and orange dulcis Compatibility 2:1 group is better than the other groups and has a significant difference.5.Determination of alcohol-induced liver injury in rat liver tissue mitochondrial membrane potential △Ψm:the model group rat liver tissue mitochondrial membrane potential △Ψm significantly lower than other groups, (P<0.01). Compatibility group transmembrane potential to be significantly lower than the single herb group that milk thistle and orange dulcis Compatibility 2:1 to set the transmembrane potential compatibility than other groups, with a significant difference. Alcoholic liver injury in rat liver tissue intracellular calcium(Ca2+) i Determination:With 50% ethanol gavage way to copy the model of alcoholic liver damage, liver tissue in model group(Ca2+) it was significantly higher than the control group, (P<0.01). Compatibility group(Ca2+) it was significantly lower than single herbs group, wherein silymarin combination Hovenia Compatibility 2:Group 1(Ca2+) i lower than other compatibility group, with significant difference.Conclusion:1.Protective effects of Semen Hoveniae silymarin and compatibility group of alcoholic liver injury in rat liver cells than single herb milk thistle and orange dulcis single flavor. Wherein Hovenia and silymarin Compatibility 2:the role of a group of the best.2.Semen Hoveniae and silymarin probably by regulating the mitochon-drial permeability transport channel, stable mitochondrial membrane potential to play a protective role on liver tissue in rats with alcoholic liver injury, to prevent liver cell apoptosis. |
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