Expression Of AQP1 And TRPV4 In The Trigeminal Ganglion Of Mice Following Stimulation Of The Oral Mucosa By Paraformaldehyde Or Hypertonic Saline

Purpose and Contents: In order to provide the morphological data for exploring the molecular mechanisms of oral sensation transmission via the trigeminal nerve, aquaporin 1(AQP1) and transient receptor potential ion channel 4(TRPV4) expression was observed in the trigeminal ganglion of mice following stimulation of the oral mucosa with paraformaldehyde or hypertonic saline.Methods: The mice were given oral submucosal injection of paraformaldehyde or oral mucosal stimulation with hypertonic saline, then immunofluorescence staining combined fluorogold(FG) retrograde tracing technique was performed to observe changes in AQP1 and TRPV4 expression in the trigeminal ganglion neurons that innervate the oral mucosa.Results: 1. Expression and distribution of AQP1 and TRPV4 in the trigeminal ganglion neurons that project to the oral mucosa was no significantly different between oral submucosal injection of paraformaldehyde-induced pain group and saline injection control group. 2. The trigeminal ganglion neurons that project to the oral mucosa increased expression of AQP1 and TRPV4 after the oral mucosa receiving hypertonic saline treatment, compared with isotonic saline control.Conclusion: Hypertonic rather than painful stimuli changes AQP1 and TRPV4 expression in the trigeminal ganglion neurons.