Hey1 Gene Is Involved In Regulating BMP9-Induced Osteogenic Differentiation Of C3H10T1/2 Cells

Objective:To analysis the effect of Heyl gene on BMP9-induced osteogenic differentiation of mesenchymal stem cells C3H10T1/2.Methods:Assemble lentivirus LV5-BMP9 and LV5-Heyl, the expression of BMP9 and Heyl detected by RT-PCR and Western blot in C3H10T1/2 cells. Then C3H10T1/2 cells were infected with LV5-Heyl and/or LV5-BMP9, the early osteogenic marker ALP activity was detected by quantitative assay, the later osteogenic marker calcium deposition was detected by Alizarin Red S staining, the cell proliferation and cycles regulated by BMP9 were detected by MTT and flow cytometry assay.Results:LV5-BMP9 and LV5-Heyl promoted the expression of BMP9 and Heyl. LV5-Heyl can increase the ealy and later osteogenic differentiation of C3H10T1/2 cells induced by BMP9, and promote the cell proliferation in the ealy stage, but inhibit cell cycles in the later stage regulated by BMP9.Conclusion:Heyl gene can influence BMP9-induced osteogenic differentiation and BMP9-regulated cell proliferation and cycles of C3H10T1/2 cells.