The Expression Of B-Catenin In Different Osteosarcoma Cells And The Effects Of B-Catenin On Cell Migration、Invasion And Osteogenic Differentiation Of TE85 Osteosarcoma Cells

ObjectiveTo detect the expression of β-catenin in 143B, MG63, and TE85 osteosarcoma cells, and the TE85 cells were infected by recombinant adenovirus to analyse the effect of β-catenin on cell migration、 invasion and of osteogenic differentiation of TE85 osteosarcoma cells.MethodsThe mRNA and protein expression of total β-catenin in different osteosarcoma cell lines were detected by RT-PCR and Western blot, and the osteosarcoma cell line TE85 was infected with Adβ-catenin, The cell migration and invasion were detected by scratch wound assay, and Transwell, respectively.and the osteosarcoma cell line TE85 was infected with Adβ-catenin,and AdBMP9, then RT-PCR and the reporter TOP-Luc were used to determine the expression of β-catenin and the the β-catenin/TCF4 activity of TE85 cells. Alkaline phosphatase (ALP) staining, ALP activity and alizarin red staining were performed to detect the early and late differention of different treated TE85 cells, respectively. Osteocalcin(OC), osteopontin(OPN), and osteoprotegerin(OPG) were detected by RT-PCR, and the protein expression level of OC and OPN were detected by western blot.Results(1). The endogenous expression of β-catenin of TE85 cells was relatively lower compared with other osteosarcoma cells (P<0.05).(2). Scratch test and Transwell results showed that the migration and invasion capacity of cells significantly decreased after Adβ-catenin infection (P<0.05).(3). The mRNA of β-catenin and BMP9 were increased by adenovirus mediated Adβ-catenin and. AdBMP9 (P<0.05), and the relative β-catenin/Tcf4 activity were increased after the infection of Ad(3-catenin(P<0.05).(4). Adβ-catenin and AdBMP9 alone infection can respectively induce ALP activity of TE85 cells at a limited level (P<0.05), but can not promote the mRNA transcriptions and protein expression of OC, OPN and OPG, as well as calcium deposition. However, both early and late osteogenic abilities were enhanced obviously after treatment with combination of Ad(3-catenin and AdBMP9 (P<0.05).ConclusionsThe endogenous expression of β-catenin of TE85 cells was relatively lower than 143B and MG63 osteosarcoma cells. The overexpression of P-catenin can effectively inhibit the migration, invasion ability of the TE85 cells. Osteogenic differentiation can be limitedly induced in TE85 cells by the overexpression of β-catenin and BMP9 alone respectively, and the osteogenesis ability is enhanced remarkably after the joint action of β-catenin and BMP9, β-catenin had a synergistic effect On the osteogenic differentiation of TE85 osteosarcoma cells.