The Role Of ASK1 In Proliferation On Injured Spinal Cord Astrocytes Of Rats

[Abstract] Objective To explore the role of apoptosis signal regulating kinase 1 (ASK1) in proliferation on injured spinal cord astrocytes of rats.Methods Get high purity spinal cord astrocytes of rats from primary culture(>98%), the injured astrocytes model in vitro was established. Injured astrocytes were immediately treated by normal saline (injured group), ASK1 antibody Anti-ASK1 antibody (Anti-ASK1 antibody group) and ASK1 inhibitor thioredo xin (Trx group). Control group were primary culture cells without anything treat. At 1h> 6h,12h、24h and 72h after injured astrocytes model was established and treated, the expression levels of GFAP and Vimentin, marker proteins of astrocytes, were detected by western blotting, the proliferative activity of astrocytes was detected by CCK-8. At 12h、24h and 72h after injured astrocytes model was established and treated, the change of the width of astrocyte scratches was detected by the immunofluorescence staining.Results At 24 and 72 hours after injured astrocytes model was established, the expression levels of GFAP and Vimentin were significantly higher in injured group compared with control group, Anti-ASK1 antibody group and Trx group (P＜0.01). At 24 and 72 hours after injured astrocytes model was established, the width of astrocyte scratches was wider in injured group compared with Anti-ASKl antibody group and Trx group (P＜0.01). Proliferative activity of astrocytes was also significantly higher in injured group compared with control group, Anti-ASK1 antibody group and Trx group at 12,24 and 72 hours after injured astrocytes model was established (P＜0.05).Conclusion Blocking ASK1 can inhibits proliferation of injured spinal cord astrocytes of rats.