Effects Of Glucagon-like Peptide-1 On Macrovascular Of Type2 Diabetic Rats

ObjectiveTo investigate the effects of glucagon-like peptide 1 on macrovascular of type2 diabetic rats and its mechanism. And then provide new ideas for individual therapy of clinical patients.Methods1.60 male SD rats were divided two groups:the normal control group (NC group) and the model group.2.The rats of model group were induced into diabetic rats by injecting streptozotocin (STZ).3.The diabetic rats were divided four roups:diabetic control group (DM group), Glibenclamide group (DMG group), Liraglutide group (DML group) and Saxagliptin group(DMS group).4.Then diabetic rats were treated with glibenclamide,liraglutide and Saxagliptin for 8 weeks while the rats of NC goup and DM group were treated with physiological saline.5. At the end of 12th week, blood was taken from abdominal aorta for examining biochemical indexes including fasting blood-glucose(FBG),fasting insulin(FIns), TC,TQLDL-C and HDL-C.The level of TNF-α was measured by ELISA.The thoracic aorta was isolated to observe the histological parameters by HE stain.6.The expression of NF-κB, ICAM-1 and VCAM-1 in aorta endothelium was determined by immunohistochemical and real time PCR.Result1.The levels of FIns,HOMA-IR,LDL-C,TG and TNF-α of GLP-1 treated rats were significantly decreased compared with glyburide group (DMG group)(P<0.05);2.The expressions of nuclear factor-KB (NF-κB), vascular cell adhesion between factor 1 (VCAM-1), and intercellular adhesion factor 1 (ICAM-1) of GLP-1 treated rats were significantly decreased compared with glyburide group (DMG group) (P<0.05);ConclusionGLP-1 can decrease the expressions of NF-κB,ICAM-1 and VCAM-1 in aorta endothelium of type2 diabetic rats. This may be involved in vascular complications of diabetes.