Determination Of Total Phenols In The Gancao Zishen Pian Tablets By UV Method

Objective:To study the preparation process of Gancao-Zishen tablets and establish a method for their quality control. Methods: The composition of tablets were designed on the basis of Microcrystalline Cellulose 、dry extract powder of Gancao and Zishen. The appearance,weight difference and disintegration time of the tablets were in line with the relevant requirements of pharmacopoeia of the People,s Republic of China(2010 edition). UV spectrophotometry was established to determine the content of total Salvianolic acid and HPLC was established to determine the content of Salvianolic acid B in 286nm;The linear range of Salvianolic acid B was 0.2412μ g~2.1708μ g,the recovery range was 98.31%~100.64%, the precision and reproducibmty was meet the request。 Results:A standard curve with liner was built up,the content of total Salvianolic acid was 11%~13%。Method of the determination for Salvianolic acid B was also built.