Protection Of Hydrogen Water On Intestine Mucosal Barrier Function In Rats With Acute Pancreatitis

Objective:To investigate the effect of hydrogen-rich water on intestine mucosal barrier function in the SD rat model of acute pancreatitis induced by retrograde intraductal injection of 5%sodium taurocholate solution.Methods:1. A rat model of acute pancreatitis was established with 45 male SD rats by retrograde intraductal injection of 5% sodium taurocholate solution. After successful modeling, rats were randomized into Groups A(sham operation), B(hydrogen-rich water)and C(normal saline).2. Laparotomy was performed in five rats from each group at 12, 24 and 36 h after modeling. Portal vein was punctured with a lancet for 2 ml of whole blood in each rat.After low-temperature centrifugation, the supernatant was aspirated. And, corresponding rats were sacrificed after sampling. Content of plasma D-lactic acid and DAO(diamine oxidase) was determined.3. Statistical analyses were performed using SPSS17.0 software. χ2 or t test was used.P<0.05 was considered as statistically significant.Results:Content of both plasma D-lactic acid and DAO in rats from hydrogen-rich water group decreased after 48 h, superior to both normal saline and sham operation groups, with a statistical difference(P<0.05).Conclusions:Hydrogen water plays a certain active role in intestine mucosal barrier function and recovery of intestinal injury in rats with acute pancreatitis, providing a new idea for linical treatment of acute pancreatitis.