Study On The Relationship Between The Persons With Occupational Pigs Contact With Carrying Staphylococcus Aureus

ObjectiveIn order to provide scientific basis for the prevention strategies and measures to control Staphylococcus aureus, we investigate the prevalence, iflucence factors of carrying, antibiotic resistant, and molecular typing of Staphylococcus aureus and understand the carriage situation of livestock-associated Staphylococcus aureus in persons with occupational pigs contact in Pearl River Delta of China.MethodsThe questionnaire survey and nasal swab sample collection were conducted in 591 occupational population and 843 control population by the convenience sampling method in Shenzhen, Dongguan, Jiangmen and Foshan. Combined with the traditional biochemical experiments and PCR method to isolate and identify Staphylococcus aureus. The antibiotic resistant of Staphylococcus aureus was used the method of Kirby-Bauer(K-B) disk diffusion to analyze. Detection of virulence genes of PVL was by the method of conventional PCR and the MRSA SCCmec typing was by the method of multiple PCR. The molecular typing of Staphylococcus aureus was by the method of multilocus sequence typing.The influence factors of carrying Staphylococcus aureus and MRSA were analyzed by using χ2 test, Fisher exact probability test and Logistic regression analysis. Results80 strains of Staphylococcus aureus(13.5%) and 43 strains of MRSA(7.5%) were isolated in occupational population. 97 strains of Staphylococcus aureus(11.5%) and 16 strains of MRSA(1.9%) were isolated in control population. The rate of MRSA carrying in occupational population was significantly higher than that of the control population(P<0.05). The drug resistance analysis found that the antibiotic resistance to 12 kinds of antibiotic drugs of MRSA was significantly higher than that of MSSA. The antibiotic resistance to 12 kinds of antibiotic drugs of Staphylococcus aureus in occupational population was higher than that of the control population. And then the rate of multiple drug resistance of Staphylococcus aureus in occupational population was higher than that of the control population. 3 strains of Staphylococcus aureus carrying PVL toxin were detected of the 177 strains of Staphylococcus aureus. The rate(1.7%) of PVL toxin carrying was low. But 3 strains of Staphylococcus aureus carrying PVL toxin were isolated from the control population. SCCmec type found the separation of MRSA is mainly IV type and V type. MLST type was found in 30 kinds of ST type. 16 strains of ST9 type Staphylococcus aureus were detected in the occupational population. The influence factors analysis showed that the risk of carrying MRSA in subjects was age. The subjects who aged below 30 years were 2.27 times than the subjects who aged above 45. The subjects who aged 30-45 years were 2.56 times than the subjects who aged above 45. Conclusions 1.The rate of MRSA carrying in occupational population was significantly higher than that of the control population. 2.The rate of Multi drug resistant of Staphylococcus aureus and the degree of resistance to Staphylococcus aureus in occupational population was higher than that of the control population. 3.The rate of Staphylococcus aureus carrying PVL toxin was low. 4.Livestock-associated Staphylococcus aureus was discovered from the occupational population. 5.The risk of carrying MRSA in subjects was related with age. 6.The genetic relationship of Staphylococcus aureus between the occupational population and the control group was far away.