Study On Effects Of Microencapsulated Olfactory Ensheathing Cells Transplantation On Neuropathic Pain Mediated By P2X_2/3 Receptors In The Dorsal Root Ganglion

Objective: To observe the therapeutic effects of microencapsulated olfactory ensheathing cells transplantation in rats with neuropathic pain. To investigate the effects and mechanisms that mediated by P2X2/3 receptors in dorsal root ganglion.Methods: 1. Nash method is used to culture olfactory ensheathing cells and the microencapsulated olfactory ensheathing cells( OECs) were made by microencapsulation technology. 2. The chronic constiction injury(CCI) rats were used as the neuropathic pain model. 3. The rats were randomly assigned into four groups:sham group(Sham); chronic constriction injury group( CCI); CCI rats treated with olfactory ensheathing cells group( CCI+OECs); CCI rats treated with microencapsulated olfactory ensheathing cells group(CCI+MC-OECs). 4. We assessed mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)in the rat models 7th and 14 th days after surgery. 5. We examined P2X2/3receptors mRNA change in L4–5 dorsal root ganglia using reverse transcriptionpolymerase chain reaction(RT-PCR)7th and 14 th days after surgery. 6. We examined P2X2/3 receptors protein change in L4–5 dorsal root ganglion using immuneohistochemistry and western-blot 7thand 14 thdays after surgery.Results: 1. CCI showed lower MWT and TWL than Sham 7th and 14 th days after surgery(P<0.05), CCI+MC-OECs and CCI+OECs showed higher MWT and TWL than Sham(P<0.05),CCI+MC-OECs showed higher MWT and TWL than CCI+OECs(P<0.05). 2. After 7th and 14 th days of surgery, the RT-PCR result of P2X2/3 receptors mRNA expression in L4–5 dorsal root ganglion indicate that: CCI was higher than Sham(P<0.05),CCI+MC-OECs and CCI+OECs were lower than CCI(P<0.05),and CCI+MC-OECs was lower than CCI+OECs(P<0.05). 3. After7 th and 14 th days of surgery, the immunohistochemistry and western-blot results of P2X2/3 receptors protein expression in L4–5 dorsal root ganglion indicate that: CCI was higher than Sham(P<0.05), CCI+MC-OECs and CCI+OECs were lower than CCI(P<0.05), and CCI+MC-OECs was lower than CCI+OECs.Conclusions: Microencapsulated olfactory ensheathing cells transplantation can suppress the neuropathic pain mediated by P2X2/3 receptors in the dorsal root ganglion.