| Purpose: Hepatocellular carcinoma(HCC) is a kind of malignant tumor with high incidence, short duration, and low survival rate, accounting for the second place of the cancer death cause, only inferior to the lung cancer. Most HCC patients have been found tumor invasion and metastasis when treatment, seriously affecting the prognosis and survival. Now there is no effective treatment for liver cancer s,molecular targeted gene therapy of malignant tumors become medical research hotspot. The aim of our study is to explore the role and mechanism of short chain enoyl-CoA hydratase 1(ECHS1) in the development of hepatocellular carcinoma cell metastasis.Methods: Firstly, we established human HepG2 cell lines and Huh7 cell lines in which ECHS1 was knocked down stably by RNA interference(RNAi) technology,detected the interference effect by Western Blot. Then detected the HepG2 cell lines and Huh7 cell lines migration in wound-healing assays and Transwell assays.To explore the migration mechanism,we detected a variety of cell migration-related genes mRNA expression in HepG2 cell ECHS1 koncked-down group and control group by RT-PCR. Finally, we detected cell migration-related protein expression levels by Western Blot.Results: We successfully established HepG2 cell lines and Huh7 cell lines in which ECHS1 was knocked down stably(HepG2-siECHS1 group and the control group HepG2-pU6, Huh7-siECHS1 group and the control group Huh7-pLV), the interference effect is significant by Western Blot(P<0.05).Wound-healing assays and Transwell assays results show the HepG2 cells and Huh7 cells metastasis ability were weakened after ECHS1 knocked down.RT-PCR and Western Blot results show the protein levels of β3-Integrin, p-FAK, p-ERK reduced after ECHS1 knocked down compared with the control group, and the difference was statistically significant(P<0.05).Conclusions: Taken together, our finding suggests that ECHS1 can promotehepatic carcinoma cell metastasis, and may be regulated by integrin signaling pathway. |
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