The Expression And Role Of Chemokine Receptor CX3CR1 In Pancreas And Associated Organ Tissues With Severe Pancretitis Rats

Objective By binding to their corresponding ligands,chemokine receptors play an vital role in the occurrence and development of severe acute pancreatits(SAP).Through establishing SAP animal model with rats,this study intends to investigate the expression of chemokine receptor CX3CR1 in pancreas and related affected organ tissues of SAP rat models, and then judge its place as a SAP predictor.Methods Sixty healthy adult male Sprague-Dawley rats,weighing200-250 g,were randomly divided into sham operation(SO)group(n=20),mild acute pancreatitis(MAP)group(n=20) and SAP group(n=20).The MAP and SAP animal models were set up by 0.5% and 5% taurocholic acid sodium respectively.Rats were put to death at 6h,12 h,24h and 48 h after molding.The pancreas tissue pathology were observed under optical microscope.The serum amylase levels in each groups were detected by rate method.Enzyme-linked immunosorbent assay(ELISA) method was used to determine the serum CX3CR1 and TNF-α levels in each groups.Meanwhile western blot and immunehistochemistry were applied to detect CX3CR1 protein expression in pancreas,lung and kidney tissues in each groups.The correlation between serum CX3CR1 and pancreas pathological damage sores,serum TNF-α levels were detected respectively.Results 1.The pancreas histopathological grading scores at each time points(6h,12 h,24h,48h) of SAP group increased significantly,compared with the according time points of SO and MAP group(P<0.05).2.The serum amylase level ateach time points of SAP group wrer higher than that of the SO and MAP group(P<0.05).3.The serum CX3CR1 and TNF-α level in SAP group,which were significantly higher than the corresponding time points of SO and MAP group, had increased since moulding,peaking at 24 h,then declined gradually(P<0.05).4.Western blot showed that the protein expression levels of CX3CR1 in pancreas and lung tissues of SAP group increased since moulding,peaking at 24h( 2.19±0.26,2.24±0.26),then declined at 48h(1.89±0.23,2.02±0.27),while that in kidney tissues increased since moulding,peaking at 48h(2.67±0.21).Compared with the corresponding time points of SO and MAP group,the protein expression levels of CX3CR1 in SAP group were significantly higher.5.Immunehistochemistry showed that CX3CR1 were expressed in pancreas,lung and kidney of SAP group,and the CX3CR1 expression levels of SAP group were significantly higher than that of SO and MAP group.Among them,the CX3CR1 expression in pancreas and lung tissue peaked at 24 h after moulding,while that in kidney tissue peaked at 48 h after moulding.6.Spearman rank correlation analysis showed: serum levels of CX3CR1 in SAP group were positively correlated with pancreas pathological damage scores(r = 0.711, P <0.01);Pearson correlation analysis showed:serum levels of CX3CR1 in SAP group were positively correlated with serum TNF-α levels( r = 0.828, P < 0.01).Conclusion 1.The chemokine receptor CX3CR1 participates in the occurrence and development process of SAP in rats,and its serum level is associatied with pancreas tissue injury severity and it might be one of the effective predictor of SAP,which deserves further research.2.The chemokine receptor CX3CR1 participates in the process of SAP associated acute lung injury in rats.3. The chemokine receptor CX3CR1 participates in the process of SAP associated acute kidney injury in rats.