Effect Of β-carotene On Intestinal Mucosal Immune Of The Immunesuppression Mice

To establish the model of immune suppression, we added β-carotene in mice diet and injected cyclophosphamide in abdominal cavity. The immune organs index, tissue section technique and Hematoxylin-Eosin staining were detected to evaluate establish the immunosuppressive model successfully. The content of serum immunoglobulin and sIgA secretion of mice intestinal mucosa was detected by ELISA kit. Immunohistochemistry was used to detect the numbers of IgA-secreting cells in mice jejunal epithelium. Semi-quantitative method to detect expression levels of TLR2 and TLR4 genes in mice intestinal mucosa. From three levels of the organization, protein, genes, we verity the impact of β-carotene on intestinal mucosal immune function of the immunosuppressed mice.From these research we came to realize that β-carotene can significantly improve the index of mice immune organ and alleviate the damaged of immune organ histology by cyclophosphamide. β-carotene can significantly increase the immuneglobulin content in serum of the immunosuppressed mice. The sIgA secretion of intestinal mucosa and the number of IgA-secreting cells were raised obviously. β-carotene can up-regulated TLR2 genes and down-regulate TLR4 genes expression in intestinal mucosa. β-carotene played a protective role in intestinal mucosa of immunosuppressed mice by activating TLR2 signaling pathway.In summary, alleviating the immunosuppression by cyclophosphamide-induced, β-carotene can protect the intestinal mucosal immune against the cyclophosphamide- induced immunosuppression. This research will lay a foundation for further study of the immune mechanism of β-carotene.