| This paper mainly studies the influence of bone marrow mesenchymal stem cells(BMSC) transplantation on cyclophosphamide(CTX)-induced ovarian injury in female rats.Method:Female rats were injected with CTX intraperitoneally(50mg/ kg on first day, then 10 mg /kg.d on the following 14 days) to induce ovarian damage. Estrus cycle was monitored by vaginal cytology at 8 every morning..Bone marrow of 14 days old SD rats was isolated, curtured and identificated for mesenchymal stem cells(MSC). And cells from 3-6 generation were used for transplantation.The experimental rats were divided into blank control group, model group and cell transplantation group. Rats of blank control group were intraperitoneally injected normal saline 1ml daily for 15 consecutive days. Rats of model group and cell transplantation group were intraperitoneally injected CTX(50mg/ kg on first day, then 10 mg /kg. d on the following 14 days) which was dissolved in 1ml normal saline. Furthermore, rats of cell transplantation group were transplantated BMSC through tail vein about 24 h after the injection of CTX.All animals were sacrificed at 1 week after stopping drug. Heart blood was taked to monitor serum concentration of E2, FSH and LH by radioimmunoassay. Ovaries were collected for other detection.Results:(1)E2 was lower but FSH and LH were higher in model group than blank control group(P < 0.05); and E2 was higher but FSH and LH were lower in cell transplantation group than model group(P < 0.05).(2)compared with blank control group, total number of follicles and number of large-follicles decreased in model group and cell transplantation group(P<0.05); but compared with model group, total number of follicles and number of large-follicles in cell transplantation group increased significantly(P<0.05).(3) Compared with blank control group, AI increased in model group and cell transplantation group(P<0.05); but compared with model group, AI significantly decreased in cell transplantation group(P<0.05).(4) Bax and bcl-2 protein exist in cytoplasm of granulosa cells of follicles. In blank control group, bax protein little exists in granulosa cells of follicles; and in model group, bax protein widely exists in cytoplasm of granulosa cells of follicles especially in the atretic follicles; and in cell transplantation group, the range of bax protein in granulosa cells is significantly less than model group. Bcl-2 protein exist estensively in the granulosa cells in blank control group; and bcl-2 protein only scattered in few granulosa cells in model group; and in cell transplantation group, the range of bcl-2 is significantly larger than model group.(5) Compared with blank control group, the expression of bax protein increased(P<0.05) and expression of bcl-2 protein decreased(P<0.05) in model group and cell transplantation group; but compared with model group, the expression of bax protein significantly decreased(P<0.05) and the expression of bcl-2 protein significantly increased(P<0.05) in cell transplantation group.Conclusion:Through this study, we found that intravenous transplantation of bone marrow mesenchymal stem cells in a certain extent reduces ovarian damage which induced by cyclophosphamide(CTX) in female rats; and further found that bone marrow mesenchymal stem cells transplantation may to reduce CTX-induced ovarian injury by regulating bax and bcl-2 protein expression levels in granulose cells.. |
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