Effect Of Microenvironment By COPD On The Proliferation,migration And Invasion Of A549 Cells

. Chronic obstructive pulmonary disease(COPD) is a preventable and treatable disease, the disease mainly consists of two parts: emphysemaand airway obstruction. Lung cancer is the most common primary malignant tumor.Recently epidemiological studies have shown that chronic obstructive pulmonary disease(COPD) is the importent risk factor associated with lung cancer, but the mechanism of the correlation is not clear. As everyone known that chronic obstructive pulmonary disease(COPD) can cause the low oxygen and high carbon dioxide, previous studies mainly focused on the effects of hypoxia on the proliferation of lung of oxygencancer cells, We establishe the cell culture model in vitro though measuring the value and carbon dioxide induced by the COPD.The experiment shows that under the condition of low oxygen and high carbon dioxide promotes the growth of A549 cells.After 24h、36h、48h of hypoxia and hypercapnia,G1/G0 phase 0f A549 decrease, with the extension of time, the ratio ofG1/G0 decrease. The S phase cells increase while an increase in VEGF expression. The proliferation of A549 cell can be promoted in the state of hypoxia and hypercapnia,which may be realized by the changes in the regulation of VEGF expressions under the hypoxic and hypercapnia condition.In the experiment we choose 5% O2 and 7% CO2 and88%N2 to culture A549 cells, The experiment shows that under the condition of low oxygen and high carbon improve the A549 cell proliferation, metastasis and invasion. And vascular endothelial growth factor(VEGF) expression increase, the low oxygen and high carbon of environment may promote the A549 cell proliferation, metastasis and invasion by increasing the expression of vascular endothelial growth factor(VEGF).