Function Of Dermis-derived Mesenchymal Stem Cells In Skin Wound Healing

Objective:Determine the role of embryoneic mice skin mesenchymal stem cells in the wound healing process,and the difference between the role of DLK1+/Sca1- and DLK1-/Sca1+ cells in the wound repair. The study will further reveal the dermal stem cells function in the process of embryonic development and explore the mechanism of action in wound healing.Methods:Skin tissue dermal stem cells will be isolated from mouse embryos through the identification of cell surface markers.Using immune magnetic bead separation technology,the DLK1+/Sca1-and DLK1-/Sca1+mesenchymal stem cells will be sorted out and the supernatant will be collected.DLK1+/Sca1-cells will be induced by multi-directional differentiation.The wound healing activity of DLK1+/Sca1- cells will be investigated by excision and incision wound healing models.Results:1. Dermal-derive stem cells by flow cytometry instrument positive expressed CD29(mesenchymal stem cell surface markers), while negative expressed CD34.2. Use the immune magnetic bead separation technology to sort of DLK1+/Sca1-and DLK1-/Sca1+ cell.DLK1+/Sca1- cell,thorough cell immunofluorescence technique, found that the positive expression of CD90, CD44.Inducting bone/fat differentiation medium,DLK1+/Sca1- and DLK1-/Sca1+ can be differentied into the direction of osteogenesis/fat cells.3. The ultraviolet spectral spectrum’s absorbing peak of supernatant is 260 nm.In the incision wound healing model, the activity of supernatant group is better than the control group.The wound healing activity of DLK1+ /Sca1- cells is better than DLK1-/Sca1+ cell group, control group and medium group in excision and incision wound healing models.The role of promoting wound healing is based on the DLK1+/Sca1- this mesenchymal stem cell.