The Effects Of Tea Polyphenol On The Oxidative Stress And Cellular Apoptosis Of Testicular Tissues In Rat Varicocele

Objective:To investigate the protective role of tea polyphenol in testicular spermatogenesis and to support theoretical and experimental basis for clinically treating varicocele with tea polyphenol through research into the effects of tea polyphenol on the oxidative stress and cellular apoptosis of testicular tissue with experimentally-induced left varicocele model in rats.Methods:Thirty-two male prepubertal Wistar rats were randomly divided into four groups.Experimental left varicocele was created by partial ligation of left renal vein(Turner method) in groups II, III and IV, and a sham operation was performed on the rats in group I.Groups I and II were fed with normal saline after four weeks postoperatively, while groups III and IV were fed with 10mg/kg(mixture concentration is 1ml/1mg/100 g with physiological saline) and 40mg/kg(mixture concentration is 1ml/4mg/100 g with physiological saline) tea polyphenol respectively, each group was intragastric administration once every day and maintained for 4 weeks. After 4 weeks all rats were executed and harvested for left testes to detect the vitality of superoxide dismutase(SOD)and the content of malondialdehyde(MDA) and to evaluate the apoptosis index(AI)respectively.Results:1.Group I represented the highest vitality of SOD, the vitality of SOD decreased in group II compared with groups III and IV(P<0.05).2.The MDA concentration in group II was higher than other groups(P<0.05).3.The AI measurement in group II was higher than other groups(P<0.05).Conclusions:1.Tea polyphenol can prominently whittle the damages of the oxidative stress and cellular apoptosis of testicular tissue with experimentally-induced left varicocele model in rats.2.Tea polyphenol can indirectly improve the testicular spermatogenesis in experimentally-induced rats.