| Objective 1. Understanding the difference of MMP-9 and TIMP-1 between normal women and PE study the relationship between PE and MMP-9, TIMP-1.Diagnosis is based on clinical data and to provide for the prevention and treatment of preeclampsia. 2. According to urinary albumin(microalbuminuri, m Alb) differentially expressed in the control group and preeclampsia urine study the relationship between the incidence of urinary micro albumin and preeclampsia. Provide early clinical evidence for predicting the occurrence of preeclampsia. 3. Understanding the relationship and difference of between m Alb and MMP-9 and TIMP-1.Method 1. Oct. 2013 ~ Aug. 2014, select the 67 cases of PE pregnant women as the observation group in Affiliated Hospital of Yan ’an University, including 32 MPE, 35 SPE, and 30 cases of normal pregnant women as control group. They are all 22~34 years old, 36~41 gestational weeks, All pregnant women were primipara, singleton pregnancies, excluded internal Medicine co-morbidities and other obstetric complications. The standard of diagnosis and classification is based on Obstetrics(Eighth Edition). "Obstetrics and Gynecology"( 8th edition, edited by Wen Li Gou) [1]. 2. After placenta tissue were dissected, embedding, section and conventional de waxing process of the 97 cases in the control group, the observation group, Detect MMP-9 and TIMP-1 ln placental tissue via IHC SP method, according to the intracellular staining intensity calculated the number of positive cells. 3. Collect selected 30 cases in the control group and 97 pregnant women with preeclampsia morning urine 5m L, after 3000 r / min and the supernatant was centrifuged 10 min, turbidimetric assay the expression of albumin in the urine in the automatic biochemical analyzer through immunization. 4. All the data were analyzed statistically by SPASS19.0(Spass19.0 statistical software). Measurement data using the t test, ANOVA(ANOVN, F test) and LSD test(data were compared between groups); count data using Pearson chi-square test(compare the overall rate), Kruskal-Wallis rank sum test(multiple sets of grades comparative data), the difference was statistically significant(P < 0.05).Result 1. MMP-9, TIMP-1 expression in the control group and preeclampsia placenta syncytiotrophoblast group cells are mainly located in the cell membrane and cytoplasm. MMP-9 in the control group and mild preeclampsia, severe group(MPE, SPE) positive expression rate of 80.0%, 71.9% and 42.9%, respectively. Comparing with each other, the difference was significant, P <0.05; TIMP-1 in normal pregnancy and preeclampsia mild, moderate and severe groups(MPE, SPE) positive rate changed little, respectively, 73.3%, 68.8% and 54.3%, compared with its pairwise difference was not statistically significant, P> 0.05; ratio becomes smaller as the expression between MMP-9 / TIMP-I, preeclampsia disease in presenting and MMP-9 similar condition changes. 2. Severe preeclampsia group maternal urine albumin testing is 421.79±73.88; mild preeclampsia group testing is 99.43±38.33; simultaneous detection of the control group is 9.41±3.49; compared with normal control group, the child m Alb value placenta preeclampsia group gradually increased with the sicker, between the two groups was significant difference(P <0.05); 3. Severe preeclampsia group, maternal age(y), gestational age(w), BMI(kg / m2), birth weight(g) were 30.9±3.6, 33.7±2.5,23.7±2.1,2845±510, eclampsia mild early maternal age group(y), gestational age(w), BMI(kg / m2), birth weight(g) of 28.5± 5.2,36.6±1.2,24.7±2.3,3084±314, respectively; while in the control group respectively, 30.5±3.5, 36.5±2.0, 23.6±2.1, 3033±351; compared among the groups, the difference was not significant, P> 0.05.Conclusion 1. Ratio smaller, In a certain extent, preeclampsia low expression of MMP-9, MMP-9 / TIMP-1,reflects the severity of preeclampsia; 2. Preeclampsia and kidney damage as a "window", microalbuminuria detection value expression may reflect the extent of early kidney disease; 3. Placenta MMP-9, change MMP-9/TIMP-1 ratio and can detect microalbuminuria as a sensitive indicator of the severity of PE assessment and diagnosis is based on clinical data provided for the prevention and treatment of preeclampsia. |
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