Isolation, Purification, Structure Characterization And Hepatoprotective Effect Of Polysaccharides From Dicliptera Chinensis

The dried aerial part of Dicliptera chinensis is one kind of both food and oriental folk medicine that has been traditionally used for years to prevent hyperpyrexia and detoxication. The effective part of Dicliptera chinensis polysaccharide(DCP) was the Mw<6000 Da group. This paper focuses on the isolation, purification, structure characterization and hepatoprotective effect of effective part of Dicliptera chinensis polysaccharide. The chief results are showed as follows:1. Purification of the effective part of DCP. The Mw<6000 Da group DCP was purified by DEAE-52 and Sephadex G-50. Molecular weight of each sub-components was determinate by gel permeation chromatography(GPC). The molecular weight are respectively: 4925 Da, 12055 Da.2. The P2 B and P4 B component structural analysis. The monosaccharide composition results showed that: P2 B contains the 5 kinds of monosaccharides: rha, ara, man, glu, gal. The main chain of monosaccharide residues of P2 B were rha, ara, man, glu, gal; the terminal residue and branch monosaccharide residue as rha, ara, gal. P2 B containing 1→6 bond type and the non-reducing end, and also containing l→2 and 1→4 bond type, two bond type ratio is about 1:1. P4 B contains the 4 kinds of monosaccharides: rha, ara, glu, gal. The main chain of monosaccharide residues of P4 B were rha, glu, gal; the terminal residue and branch monosaccharide residue as glu, rha, ara, gal. P4 B also containing two bond type, two bond type ratio is about 5:6.3. The protective effects of P2 B and P4 B on L-02 cell line injury induced by CCl4. The chief results are showed as follows:(1) The L-02 cell viability was 60%-70% when it was induced by 6mmol/L CCl4, so we establish the stable liver injury model induced by 6mmol/L CCl4.(2) The cell viability in model group reduced markedly(62.16±1.62 %), P2B(0.5、0.25、0.125 mg/m L) and P4B(0.5、0.25、0.125 mg/m L) can enhance cell viability evidently(P<0.01), The protective effect enhanced with the increase of concentration of P2 B and P4 B.(3) Compared with normal group, ALT and AST levels of model group were significantly higher(P<0.01); Compared with model group, every concentration of P2 B and P4 B could reduce the elevated ALT and AST levels. P2B(0.5、0.25 mg/m L) and P4B(0.5、0.25mg/ml) could reduce ALT and AST levels((P<0.01).(4) Compared with normal group, MDA level of model group were significantly higher(P<0.01), meanwhile the activity of SOD were reduced(P<0.01), P2B(0.5、0.25、0.125 mg/m L) and P4B(0.5、0.25、0.125 mg/m L) could reduce the levels of MDA(P<0.01), P4B(0.5、0.25 mg/m L) could enhance the activity of SOD(P<0.05).