Study On The Quality Standard Improvement Of Lancong Tea

Objective:To mature the quality control measures of Lancong Tea and provide a scientific basis for further study on it, the topic researched on improvement in Lancong Tea original quality standard and established its HPLC fingerprint. Methods:(1) Adopted thin layer chromatography(TLC) to identify four kinds of Chinese herbal medicines in the recipe, and established the TLC identification methods of them.(2) Adopted high performance liquid chromatography(HPLC) to determine the contents of rosmarinci acid and gallic acid, and established HPLC methods for determination the content of them.(3) Adopted HPLC to study the fingerprint of Lancong Tea, and established its HPLC fingerprint. Results:(1) Established the TLC differential conditions of Canarii Fructus,Perillae Folium and Zingiberis Rhizoma Recens and the TLC spots were clearly and separated well, and the negative control did not impose any disturbance.(2) Determined the HPLC conditions of the content of rosmarinci acid and gallic acid respectively: the analysis was carried out on Inertsil○R ODS-C18 column(4.6mm× 150 mm,5μm), mobile phase was methanol-0.1%phosphonic acid(35:65) with flow rate of 1.0 m L/min isocratic elution, detection wave length was 330 nm, the number of theoretical plates is calculated by rosmarinic acid peak of not less than 6000;while the analysisofgallicacidwascarriedoutoncapcellpark-c18column(4.6mm×250mm,5μm),mobilephasewasmethanol-0.1%phosphonicacid(5:95)withflowrateof1.0ml/minisocraticelution,detectionwavelengthwas216 nm,thenumberoftheoreticalplatesiscalculatedbygallicacidpeakofnotlessthan3000.(3)determinedthehplcconditionsofthefingerprintanalysisoflancongtea,andthechromatographicconditionswereasfollows:inertsil○rsod-c18(250mm×4.6mm,5μm)column;mobilephaseconsistedofmethanol-0.1%phosphorousacidsolution,withratioofmethanol:0~15min:5~15%,15~43min:15~45%;43~55min:45~62%;55~80min:62%(gradientelution);thedetectionwavelengthat273nm;thecolumntemperatureof20℃;theflowrateof1.0ml·min-1,gallicacidpeakasthereferencepeak,andestablishedthehplcfingerprintoflancongtea.conclusion:improvedthequalitycontrolstandardoflancongtea,andthetlcdifferentialmethodsandthedeterminationmethodsofthecontentsofrosmarinciacidandgallicacidwerespecific,sensitiveandrepeatable,canbeusedtocontrolthequalityoflancongteabetter.thehplcfingerprintoflancongteawassuitforitsqualitycontrol,andcanprovideascientificbasisforfurtherstudyonit.