Correlation Study Of Muscone On Osteogenic Differentiate On Of Human Periodontal Ligamentstem Cells

The destruction of dental caries, periodontal disease and irreversible periodontal tissue trauma lead to tooth defects and seriously affect the People’s lives. Therefore, Dental scientists committed to curing the disease by tissue engineering technology.Periodontal ligament stem cell derived from the adult stem cells of periodontal ligament, With a strong self-renewal capacity, the cell surface marker protein is similar with BMSCs, hPDLSCs can differentiate into a variety of cells with different functional properties. The study showed that hPDLSCs maintain periodontal ligament’s function stability, repair damaged tooth tissue and promote functional cell renewal etc. hPDLSCs is the most important to periodontal tissue regeneration. Besides, hPDLSCs is the basis of periodontal tissue disease therapy and gene therapy mode, therefore, hPDLSCs has broad prospect in application of tissue engineering and regenerative medicine. Studies have proposed that musk ketone promotes BMSCs proliferation and osteogenic differentiation in rats, but there are no researches of osteogenic differentiation on PDLSCs. Therefore, we evaluate the influence musk ketone on osteogenic differentiation of hPDLSCs through in vitro cell viability and osteogenic differentiation, so as to provide a theoretical basis of hPDLSCs in prosthodontics.Objective:To isolated and purified PDLSCs, musk ketone action by a certain concentration of PDLSCs after a certain time, to detect the level of transcription and expression levels PDLSCs bone-related genes to change and to study the musk ketone can promote PDLSCs osteogenic differentiation.Methods:freshly third molar with healthy periodontal coming from clinical orthodontic removal is collected, isolated and cultured periodontal ligament stem cells by enzyme digestion combined with limited dilution method, through flow cytometry to identify cell surface marker protein of periodontal ligament stem cells.1. The periodontal ligament stem cells were seeded into 96-well plates with density of 1×103cell/well, treated with a concentration of musk ketone role 3mg/L, after 0,24 and 48h, using CCK-8 to detect the levels of cell proliferation. The PDLSCs secretion levels of bone morphogenetic protein were measured by ELISA.2. mRNA levels were measured the changing of bone morphogenetic protein in transcription of genes, evaluation of musk ketone osteogenic differentiation of human periodontal ligament stem cells through detecting alkaline phosphatase activity.Results:Musk ketone promotes human periodontal ligament stem cell proliferation, promotes secretion of bone morphogenetic protein-2 and bone morphogenetic protein-7 after 48h, promotes mRNA transcription of osteoblast gene Runx2, osteocalcin, osteopontin and cementum attachment protein, and alkaline phosphatase activity was significantly higher than control group.Conclusions:Musk ketone promotes osteogenic differentiation of human periodontal ligament stem cells in vitro.