Pharmacodynamic Study Of Trichosanthes Pericarpium On Acute Myocardial Ischemia In Rats

Objective: Using different extraction process to separate different parts of the chemical constituents of Trichosanthes Pericarpium, copy the model of acute myocardial ischemia by ligating the left anterior descending coronary artery of rats, evaluate different chemical parts and components’ intervention effect of coronary artery disease by studying the change of enzyme activity of creatine-kinase(CK), creatine-kinase-MB(CK-MB), lactate-dehydrogenase(LDH), α-Hydroxybutyrate Dehydrogenase(α-HBDH) and aspartate-amino transferase(AST) in the model of rat plasma, Preliminary explore the active ingredient or parts of Trichosanthes Pericarpium for the treatment of coronary heart diease, which provide a basis for the Breeding of fine varieties draught of quality standards and quality control of Trichosanthes Pericarpium injection.Methods: Wistar male rats were randomly divided into the control group, model group, positive control group, the Trichosanthes group. To make acute myocardial ischemia animal model, the left anterior descending coronary branch were ligated in male Wistar rats that have received intragastrical administration for 4 days. drug intervention for model rat by the water extraction, alcohol extraction and Guanosine that are all from Trichosanthes Pericarpium.6h after of operation, myocardial tissue was stained with triphenyltetrazolium chloride(TTC) to calculate the infarction rate. To evaluate the myocardial injury, serum levels of creatine-kinase(CK), creatine-kinase-MB(CK-MB), lactate dehydrogenase(LDH),α-Hydroxybutyrate Dehydrogenase(α-HBDH) and aspartate-amino transferase(AST) were detected in these different groups of rats.Results : ①After precipitation of water decoction by alcohol solvent, we get the water extract that contains a lot of nucleoside compounds, which participate in the generation of the body’s metabolism and material life. Experiments show that water extracts has significant therapeutic effect on acute myocardial ischemia rats. ②Alcohol extract was conducted with a certain concentration of ethanol contains palmitic acid, alpha spinach sterol glycosides, bean ene alcohol and the composition such as glucose inside. The experiment proves the alcohol extract has no significant effect on the treatment of acute myocardial ischemia in rats. ③After the water extract-alcohol precipitation of the Trichosanthes Pericarpium, by using macroporous adsorption resin separation of coarse type combined with half preparation HPLC purification for it, concluded the monomer chemical composition, we get guanosine. The animal experiments in vitro preliminary thought to the intervention of acute myocardial ischemia of rats effect is not obvious.Conclusions: The Trichosanthes Pericarpium water extract can significantly reduce the rate of acute myocardial ischemia model of rat myocardial infarction, reduce the release of myocardial enzymes, ischemia of rat myocardium has obvious protective effect. Trichosanthes Pericarpium alcohol extract can reduce the myocardial enzyme activity on acute myocardial ischemia rats, but the effect is not obvious. Guanosine monomer is extracted from Trichosanthes Pericarpium water extract, has no significant protective effect of ischemic myocardium in rats.