Specifically Labeled Midbrain Dopaminergic Neurons By CRISPR/Cas9n System

Parkinson disease (PD) is a relatively common neurological diseases that can gives a series of serious impact on human life, and no cure at present. The main pathological hallmark of PD is the progressive disappearance of tyrosine hydroxylase (TH)-expressing dopaminergic(DP) neurons in substantia nigra pars compacta (SNpc). Cell therapy is the most promising way to improve the quality of PD patients’life, significantly, however when we starting cell therapy clinical or cure PD exhaustive, we’d better to find a way to comprehensive understand the cells we achieved. Although a number of rodent model has been established for PD research, a small amount of DP neorons can be get in vitro and even DP neurons can be using hTH promoter specifically labeled in mice, but we has not yet been able to mark and obtain pure human DP neurons for depth studying, which hinder the process of studying DP neurons comprehensive. To aid in these problem, in Non-xenogeneic animal ingredients conditions, the recent discovery gene editing system ----CRISPR/Cas9n system was used, which is more efficiency, ease of operation, low cost and more accurate. Finally, a hES cell line carrys in vitro cloning short tyrosine hydroxylase promoter-driven ZsGrenn has been successfully established, and then specifically labeled midbrain dopaminergic neurons induced from that cell line. Thus, these work laid the roots for more detailed analysis of midbrain DA neurons in the following work.