Research About Antibacterial Activity Of Extractive And Effective Ingredients From Artemisia Capillaris

Research objectives: Artemisia capillaris is a kind of herbaceos perennial which belongs to Compositae. It is the primary drug to treat jaundice with the effect of heat and dampness or transaminase. Artemisia capillaris is mainly used for clinical treatment of jaundice and oliguria, wet sores itching,infectious jaundice hepatitis. In recent years, there are many articles about jaundice of Artemisia capillaris, relativey speaking, the antimicrobial or antitumer report is few related, This topic mainly extract and isolate the constituents from Artemisia capillaris guided by antimicrobial activity to offer a scientific basis for developing new antimicrobial drugs from Artemisia capillaris.Research Methods: This article uses staphylococcus aureus and streptococcus mutans as test bacteria to confirm antimicrobial activity of petroleum ether layer and ethyl acetate layer.Guided by antimicrobial activity for kinds of bacteria,we seperate and purify the monomer compounds with sorts of Chromatographic methods We apply the double dilution method in succession to test Minimal inhibitory concentration(MIC) of Streptococcus mutans,Staphylococcus aureus, B. subtilis and E. coli. If the seperated compounds have excellent antibacterial activity,we continue to conduct an experiment of minimal bactericidal concentration(MBC) to test drug resistance.simultaneously, we test the radical-Scavenging activity of monomer compounds according to the common method of DPPH to confirm antioxidative activity.Eventually,a experiment of the effect for human cervical cell (hela) to verify whether the pure compounds have antitumor activity is conducted.Research results: After repeated column,we seperate seven compounds in the end,and apply modern spectrum techniques such as nuclear magnetic resonance(NMR)(1H-NMR,13C-NMR,MS) to confirm their structures,They are compound Ⅰ(β-sitosterols),compound Ⅱ (hexadecanoicacid),compoundⅢ(azelaic acid),compoundⅣ(ferulicacid),compoundⅤ(quercetin),compoundⅥ(chlorogenicacid) and compound Ⅶ (luteolin).The MIC result for Staphylococcus aureus,Streptococcus mutans,E.Coli and B. subtilis of compoundⅢ,Ⅳ,Ⅵ and Ⅷ is 8～64μg/mL,and compound Ⅰ and compound Ⅱ have no effect for the test bacteria.It is first reported that compound Ⅴ has antimicrobial activity against Pseudomonas aeruginosa ,Bacillus cereus,and E. Coli.lt is also the first time that we know compound Ⅶ could kill pseudomonas aeruginosa .The consequence of minimal bactericidal concentration(MBC) shows that the data of MBC is greater than the data of quadruple MIC for compound Ⅴ against test bacteria.so it is known that compound Ⅴ is reversible antibacterial and is susceptible to multidrug resistance.However,compound Ⅳ,compoundVI an d compoundⅦ only have multidrug resistance for individual bacteria. According to the radical-Scavenging of DPPH research findings,we may safely draw a conclusion that compoundIV,compoundVI and compoundⅦ display strong antioxidation capacity.In the meantime, the effect for human cervical cell (hela) turned out that compoundⅢ,Ⅶ can anti-cancer significantly.