3D-printed Alginate/Hydroxyapatite Hydrogel With Atsttrin For Bone Defects Repair

In many fracture and bone defect cases, the patients are under local or systemic inflammatory conditions. TNF-a, one of the inflammatory cytokines, delays the healing process. It was reported that overexpression of TNF-a enhanced bone resorption and inhibited osteogenesis induced by BMP-2. Applying antagonist of TNF-a to biodegradable materials would help to improve the bone repair, thus bring us a new strategy for bone tissue engineering. It has been shown that PGRN and its derived protein, Atsttrin, could interrupt the effect of TNF-a by interacting with its receptor. Therefore, we hypothesize that implantation of Atsttrin-incoporated biodegradable scaffold would facilitate bone repair. In this study, a3D bioprinting system was used to fabricate Atsttrin-Alg/nHAp composite scaffolds, and Atsttrin release from this scaffold was chracterized, followed by evaluation of its efficacy on bone regeneration both in vitro and in vivo. In summary, this3D-printed Atsttrin-Alg/nHAp scaffold with precise structure and anti-inflammatory property could be promising for bone defect repair.