The Different Expression Of Cathepsin D In Glioma And Astrocyte

Background and Objective:One of the reason for glioma poor prognosis lies in the invasive glioma growth. Finding an effective way to block glioma intracranial invasive growth is of great significance. Glioma lysosome enzyme Cathepsin D could play an important role in glioma invasion. To further study the role of the Cat D, we compared the Cat D expression and exocytosis in rat astrocytes, rat glioma cell line and human glioma cell line. The research makes a basis for the future experimental glioma treatment.Methods:Within the primary culture rats astrocytes, rat glioma cell line and human glioma cell line, the Cat D content was detected by Western blot. The different Cat D distribution in the early endosome and late endosome and lysosome was observed by immunocytochemistry within the three cell types. The Cat D release was checked by ELIS A within the three cell types.Results:There are more mature Cathepsin D content in astrocytes compared with the contend in glioma cells. There are no difference for the Cathepsin D distribution in the early endosome and lysosomes between astrocytes and glioma cells. Astrocytes release more Cathepsin D compared with the glioma cells. Conclusion:The content of Cathepsin D is different from glioma cells and astrocytes, while the distribution of Cathepsin D has no difference between glioma cells and astrocytes. Astrocytes release more Cathepsin D compared with glioma cells.