The Expression And Fuction Of NR5A1/NR5A2in The Decidual Tissus Of Patients With Preeclampsia

Preeclampsia is a multisystem disorder unique to human, which causes maternal andperinatal mortality and morbidity. It affects5to8%of all pregnancies and is responsible forapproximately50,000maternal deaths annualy. Preeclampsia is a potentially dangerouscomplication of the second half of pregnancy, labor, or early period after delivery, characterizedby hypertension, abnormal amounts of protein in the urine, and other systemic disturbances. Thepathogenesis of preeclampsia is still mysterious, but it is mostly multifactorial. Abnormaldecidualization may cause preeclampsia. The endometrium undergoes cyclical changes includingproliferation, differentiation, and changes into decidual cells. This process, termeddecidualization, is crucial for embryo implantation and maintenance of the pregnancy.Progesterone-and cAMP-mediated signaling pathways act key roles in the induction andmaintenance of the decidual phenotype and function. There are also many important signalingmolecule in progesterone-and cAMP-mediated signaling pathways, such as, transcription factorC/EBPβ, Hoxa-10, BMP2, and Wnt4.NR5A1and NR5A2belong to orphan nuclear receptor family, which is one of the threemembers of the nuclear receptors. In has been found that some of the orphan nuclear receptorsare involved in animal metabolic regulation, embryonic development, cell differentiation, andgene expression. NR5A1and NR5A2are involved in animal follicular development, femalereproduction, and steroidogenesis. It is known that NR5A2is essential for human decidualization,so do NR5A1and NR5A2both act roles in decidualization? Is it possible that abnormalexpression of NR5A1or NR5A2may lead to preeclampsia?Our data demonstrated that NR5A2was upregulated after in vitro decidualization, butNR5A1expression was low and has no difference compared with control. It Indicated thatNR5A2may involved in decidualization while NR5A1may not. After knocking down ofNR5A2in hESCs resulted in a significant reduction in the expression of mRNAs corresponding to IGFBP-1and PRL. This verified again that NR5A2may play a role in decidualization. Thestudy on decidual tissus showed that the level of NR5A2mRNA and protein expression ofpatients with severe preeclampsia was lower than that of normal pregnant people, while NR5A1expression showed no significant difference.So we speculated that the disorder of NR5A2in endometrium may associated withabnormal decidualization which may cause preeclampsia.