The Role And Mechanism Of Intervention Shenmai Injection On Human Embryonic Lung Fibroblast

Objective: Through the observation about the influence that shenmai injection to the proliferation and apoptosis of human embryonic lung fibroblast and secretion of related cytokine which induced by Transforming growth factor beta 1(TGF-beta 1), We evaluate the efficacy of shenmai injection on pulmonary fibrosis.Methods: Order human fetal lung fibroblasts as the research object were randomly divided into five groups:normal control group, model group, shenmai injection low-dose group, shenmai injection medium-dose group and shenmai injection high-dose group, adding the appropriate reagent to intervene in accordance with the grouping and training is completed, To detect the stimulation of human fetal lung fibroblasts in different group by MTT assay. And flow cytometry Annexin V / PI double staining was used to checkapoptosis rate. Semi-quantitative RT-PCR was used to check the content of collagen Ⅰ(COL-Ⅰ) mRNA, collagen Ⅲ(COL-Ⅲ) mRNA, α- smooth muscle actin(α-SMA) mRNA, extracellular superoxide dismutase(ECSOD) mRNA and platelet-derived growth factor(PDGF) mRNA; ELISA was used to detected the level of COL-Ⅰ, COL-Ⅲ, α-SMA, ECSOD, PDGF protein.Results: Model group proliferation the absorbance(OD) value is greater than the control group, there was a significant difference(P <0.05); Shenmai injection cell proliferation the absorbance(OD) values were lower than the model group, there was a significant difference(P <0.05), SMI was high, medium and low concentrations of two groups were significantly different(P <0.05), the higher the concentration, the smaller the OD. Model group apoptosis rate than the control group, there was a significant difference(P <0.05); Shenmai injection group apoptosis rate higher than the model group, there was a significant difference(P <0.05), Shenmai injection high, medium and low concentrations of two groups were significantly different(P <0.05), the drug concentration, the higher the rate of apoptosis. Model cells COL-Ⅰ, COL-Ⅲ, α-SMA, PDGF expression was higher, there was a significant difference(P <0.05); Shenmai injection group were COL-Ⅰ, COL-Ⅲ, α-SMA, PDGF expression levels lower than the model group, there was a significant difference(P <0.05), SMI was high, medium and low concentrations of two groups were significantly different(P <0.05), the higher the drug concentration, The lower expression. ECSOD model group than the control group, the expression of cell, there was a significant difference(P <0.05); Shenmai injection group were ECSOD expression were higher than the model group, there was a significant difference(P <0.05), Shenmai injection high, medium and low concentrations of two groups were significantly different(P <0.05), the drug concentration, the higher the expression.Conclusion : Shenmai injection has the effect of by TGF- β 1-induced human embryonic lung fibroblasts proliferation and promoting apoptosis, Shenmai injection could inhibit the synthesis of collagen in lung fibroblasts and the transformation of fibroblast cells into myofibroblasts. It has the effect of anti-oxidation and reduce level of platelet-derived growth facto. Within a certain range, the higher of the drug concentration, the stronger effect it has.